Recombinant human ACE-2 protein | NKMAX ATGP3963 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human ACE-2 protein

catalog :

ATGP3963

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human ACE-2 protein

Domain :

18-740aa

Catalog number (SKU) :

ATGP3963

NCBI :

NP_068576.1

Description :

ACE-2, also known as Angiotensin-converting enzyme 2, is an enzyme attached to the cell membranes of cells in the lungs, arteries, heart, kidney, and intestines. This enzyme lowers blood pressure by catalysing the hydrolysis of angiotensin II into angiotensin. It counters the activity of the related angiotensin-converting enzyme (ACE) by reducing the amount of angiotensin-II and increasing Ang(1-7) making it a promising drug target for treating cardiovascular diseases. ACE-2 also serves as the entry point into cells for some coronaviruses, including HCoV-NL63, SARS-CoV, and SARS-CoV-2. Recombinant human ACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques

Alternative Names :

Angiotensin-converting enzyme 2, ACEH, Angiotensin-converting enzyme-related carboxypeptidase, ACE-related carboxypeptidase, Metalloprotease MPROT15, ACE2

Concentration :

0.5mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hamming I., et al, (2004) J Pathol. 203:631-637.;; Mohammed A.r. Chamsi-Pasha., et al, (2014) Curr Heart Fail Rep. 11:58-63.

Purity :

> 90% by SDS - PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with SARS-CoV-2 Spike RBD. The ED50 range ≤ 100ng/ml. Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH per minute at pH 7.5, at 25C.

Applications :

SDS-PAGE, Enzyme Activity, Bioactivity

Antigen Species :

Human

Gene Category :

Virus

Molecular Weight :

110.8kDa (965aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

SARS-CoV-2 Spike RBD is coated at 5 ug/ml (100 ul/well) can bind Human ACE-2. The ED50 range ≤ 100ng/ml.

Amino Acid Sequence :

QSTIEEQ AKTFLDKFNH EAEDLFYQSS LASWNYNTNI TEENVQNMNN AGDKWSAFLK EQSTLAQMYP LQEIQNLTVK LQLQALQQNG SSVLSEDKSK RLNTILNTMS TIYSTGKVCN PDNPQECLLL EPGLNEIMAN SLDYNERLWA WESWRSEVGK QLRPLYEEYV VLKNEMARAN HYEDYGDYWR GDYEVNGVDG YDYSRGQLIE DVEHTFEEIK PLYEHLHAYV RAKLMNAYPS YISPIGCLPA HLLGDMWGRF WTNLYSLTVP FGQKPNIDVT DAMVDQAWDA QRIFKEAEKF FVSVGLPNMT QGFWENSMLT DPGNVQKAVC HPTAWDLGKG DFRILMCTKV TMDDFLTAHH EMGHIQYDMA YAAQPFLLRN GANEGFHEAV GEIMSLSAAT PKHLKSIGLL SPDFQEDNET EINFLLKQAL TIVGTLPFTY MLEKWRWMVF KGEIPKDQWM KKWWEMKREI VGVVEPVPHD ETYCDPASLF HVSNDYSFIR YYTRTLYQFQ FQEALCQAAK HEGPLHKCDI SNSTEAGQKL FNMLRLGKSE PWTLALENVV GAKNMNVRPL LNYFEPLFTW LKDQNKNSFV GWSTDWSPYA DQSIKVRISL KSALGDKAYE WNDNEMYLFR SSVAYAMRQY FLKVKNQMIL FGEEDVRVAN LKPRISFNFF VTAPKNVSDI IPRTEVEKAI RMSRSRINDA FRLNDNSLEF LGIQPTLGPP NQPPVS

Tag :

hIgG-His-Tag

Expression System :

HEK293

Predicted nterm :

Asp

Product Type Name :

Recombinant Protein

UniProt No. :

Q9BYF1

Internal Search Terms :

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more info or order :

NKMAX product webpage