protein

Recombinant SARS-CoV Spike RBD protein

ATGP3960

10 ug

150 USD

Recombinant SARS-CoV Spike RBD protein

306-515aa

ATGP3960

NP_828851.1

Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), and the recently identified novel Coronavirus (SARS-CoV-2) belong to the Coronaviridae family, genus Betacoronavirus, that has been related to important epidemiological outbreaks. SARS-CoV emerged in 2003 as a significant threat to human health. SARS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. It attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit. Recombinant SARS-CoV spike RBD protein fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

E2 glycoprotein precursor, Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Severe acute repiratory Syndrome-related Coronavirus, SARS, SRAS-CoV, SARS-CoV1

0.5 mg/ml (determined by Absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kukla M., et al, (2020) J Clin Med. 9:1420.;; Ayouba A., et al,(2020) J Clin Virol. 129:104521.;; Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93-116.;; Li F, et al, (2005) Science. 309:1864-1868.;; Struck AW, et al, (2012) Antiviral Res. 94:288-296.

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# ATGP3963)

SDS-PAGE, Bioactivity

SARS-CoV

Virus

24.7kDa (219aa)

< 1 EU per 1ug of protein (determined by LAL method)

SARS-CoV Spike RBD is coated at 5ug/ml (100 ul/well) can bind ACE-2 (CAT# ATGP3963) in a Functional ELISA assay.

RVVPSGD VVRFPNITNL CPFGEVFNAT KFPSVYAWER KKISNCVADY SVLYNSTFFS TFKCYGVSAT KLNDLCFSNV YADSFVVKGD DVRQIAPGQT GVIADYNYKL PDDFMGCVLA WNTRNIDATS TGNYNYKYRY LRHGKLRPFE RDISNVPFSP DGKPCTPPAL NCYWPLNDYG FYTTTGIGYQ PYRVVVLSFE LLNAPATVCG PKL

His-Tag

Baculovirus

Ala

Recombinant Protein

P59594

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