protein

Recombinant human Osteopontin/OPN protein

ATGP3951

10 ug

60 USD

Recombinant human Osteopontin/OPN protein

17-314aa

ATGP3951

NP_001035147

Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

OPN, SPP-1, BNSP, BSPI, ETA-1, Bone sialoprotein 1, BSP I.Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Secreted phosphoprotein 1, SPP 1, SPP1, urinary stone protein, uropontin,

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 2mM EDTA

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Koh A., et al. (2007). Immunology. 122(4):466-75;; Sodek J., et al. (2006). J Dent Res. 85(5):404-15;;

> 90% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to OPN coated plates 10ug/ml. This effect is more to 40%.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

36.2 kDa (321aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

< 1 EU per 1ug of protein (determined by LAL method)

Human OPN stimulates cell adhesion of the HEK293 human embryonic kidney cells. This effect is more to 40%.

RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N

His-Tag

E.coli

Recombinant Protein

P10451

Osteopontin, OPN, OPN, SPP-1, BNSP, BSPI, ETA-1, Bone sialoprotein 1, BSP I.Early T lymphocyte activation 1, ETA 1, ETA1, MGC110940, Nephropontin, Secreted phosphoprotein 1, SPP 1, SPP1, urinary stone protein, uropontin,ATGP3951-10ug,ATGP3951-20ug,ATGP3951-50ug,ATGP3951-100ug,ATGP3951-250ug,ATGP3951-500ug,ATGP3951-1mg,ATGP3951-10,ATGP3951-20,ATGP3951-50,ATGP3951-100,ATGP3951-250,ATGP3951-500,ATGP3951-1, ATGP0274

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012){https://pubmed.ncbi.nlm.nih.gov/22843993/};;Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016){https://pubmed.ncbi.nlm.nih.gov/27040940/};;Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017){https://pubmed.ncbi.nlm.nih.gov/28536714/};;Kim ID, et al. Osteopontin Peptide Icosamer Containing RGD and SLAYGLR Motifs Enhances the Motility and Phagocytic Activity of Microglia. (Exp Neurobiol. 2017){https://pubmed.ncbi.nlm.nih.gov/29302201/};;Jung SH, et al. On-chip dual enzyme activity assay to investigate regulation of the transamidase and kinase activities of transglutaminase 2. (Anal Chim Acta. 2018){https://pubmed.ncbi.nlm.nih.gov/29866275/};;Jung SH, et al. Array-based Investigation of Amino Acids Responsible for Regulation of Transamidase and Kinase Activities of Transglutaminase 2. (BioChip J. 2019){https://doi.org/10.1007/s13206-019-3307-3}