Recombinant human TIE2/TEK protein | NKMAX ATGP3947 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human TIE2/TEK protein

catalog :

ATGP3947

quantity :

10 ug

price :

110 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human TIE2/TEK protein

Domain :

23-748aa

Catalog number (SKU) :

ATGP3947

NCBI :

NP_000450

Description :

TEK, also known as angiopoietin-1 receptor isoform 1, is an endothelial cell-specific receptor tyrosine kinase subfamily (RTK). This protein acts as cell-surface receptor and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels and is required for normal angiogenesis and heart development during embryogenesis. Recombinant human TEK protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

TEK, TEK receptor tyrosine kinase, VMCM, venous malformations, multiple cutaneous and mucosal, TEK tyrosine kinase, endothelial, TIE2, TIE-2, VMCM1, CD202b, angiopoietin-1 receptor, Hyk, AA517024, STK1, p140 TEK

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kitajima D., et al, (2016) J Cancer. 7:600-607.;;Kelly-Goss MR., et al, (2017) Sci Rep. 7:9049.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human Angiopoietin-2 (CAT# ATGP3853). The ED50 range ≤1 ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Signal Transduction

Additional Comment Only :

ATGP3762 has been replaced with a catalog number ATGP3947.

Molecular Weight :

107.9 kDa (965aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human Angiopoietin-2 (CAT# ATGP3853) is coated at 5 ug/ml (100 ul/well) can bind Human TIE-2. The ED50 range ≤1 ug/ml.

Amino Acid Sequence :

AMDLILINSL PLVSDAETSL TCIASGWRPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGEAIR IRTMKMRQQA SFLPATLTMT VDKGDNVNIS FKKVLIKEED AVIYKNGSFI HSVPRHEVPD ILEVHLPHAQ PQDAGVYSAR YIGGNLFTSA FTRLIVRRCE AQKWGPECNH LCTACMNNGV CHEDTGECIC PPGFMGRTCE KACELHTFGR TCKERCSGQE GCKSYVFCLP DPYGCSCATG WKGLQCNEAC HPGFYGPDCK LRCSCNNGEM CDRFQGCLCS PGWQGLQCER EGIPRMTPKI VDLPDHIEVN SGKFNPICKA SGWPLPTNEE MTLVKPDGTV LHPKDFNHTD HFSVAIFTIH RILPPDSGVW VCSVNTVAGM VEKPFNISVK VLPKPLNAPN VIDTGHNFAV INISSEPYFG DGPIKSKKLL YKPVNHYEAW QHIQVTNEIV TLNYLEPRTE YELCVQLVRR GEGGEGHPGP VRRFTTASIG LPPPRGLNLL PKSQTTLNLT WQPIFPSSED DFYVEVERRS VQKSDQQNIK VPGNLTSVLL NNLHPREQYV VRARVNTKAQ GEWSEDLTAW TLSDILPPQP ENIKISNITH SSAVISWTIL DGYSISSITI RYKVQGKNED QHVDVKIKNA TITQYQLKGL EPETAYQVDI FAENNIGSSN PAFSHELVTL PESQAPADLG GGKMLL

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala23

Product Type Name :

Recombinant Protein

UniProt No. :

Q02763

Internal Search Terms :

Tie2, Angiopoietin-1 receptor isoform 1, TEK, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1, ATGP3947-10ug, ATGP3947-20ug, ATGP3947-50ug, ATGP3947-100ug, ATGP3947-250ug, ATGP3947-500ug, ATGP3947-1mg, ATGP3947-010, ATGP3947-020, ATGP3947-050, ATGP3947-100, ATGP3947-250, ATGP3947-500, ATGP3947-01M, ATGP3762

more info or order :

NKMAX product webpage