Recombinant Human ACE Protein | NKMAX ATGP3944 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant Human ACE Protein

catalog :

ATGP3944

quantity :

10 ug

price :

140 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant Human ACE Protein

Domain :

30-1256aa

Catalog number (SKU) :

ATGP3944

NCBI :

NP_000780

Description :

ACE, also known as peptidyl-dipetidase A, is a zinc metallopeptidase important for blood pressure control and water and salt metabolism. it converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. It's able to inactivate bradykinin, a potent vasodilator. It has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Recombinant human ACE/CD143, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Angiotensin-coverting enzyme, Dipeptidyl carboxypeptidase I, Kininase II, ACE1, CD143, DCP, DCP1

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Amati E., et al, (2018) Stem Cell Res Ther. 9:10.;;Metzger R., et al, (2000) Atherosclerosis. 150:21-31.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK(Dnp)-OH per minute at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cardiovascular

Molecular Weight :

142kDa(1235aa)

Endotoxin :

<1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK(Dnp)-OH per minute at 25C.

Amino Acid Sequence :

LDPGLQPGNF SADEAGAQLF AQSYNSSAEQ VLFQSVAASW AHDTNITAEN ARRQEEAALL SQEFAEAWGQ KAKELYEPIW QNFTDPQLRR IIGAVRTLGS ANLPLAKRQQ YNALLSNMSR IYSTAKVCLP NKTATCWSLD PDLTNILASS RSYAMLLFAW EGWHNAAGIP LKPLYEDFTA LSNEAYKQDG FTDTGAYWRS WYNSPTFEDD LEHLYQQLEP LYLNLHAFVR RALHRRYGDR YINLRGPIPA HLLGDMWAQS WENIYDMVVP FPDKPNLDVT STMLQQGWNA THMFRVAEEF FTSLELSPMP PEFWEGSMLE KPADGREVVC HASAWDFYNR KDFRIKQCTR VTMDQLSTVH HEMGHIQYYL QYKDLPVSLR RGANPGFHEA IGDVLALSVS TPEHLHKIGL LDRVTNDTES DINYLLKMAL EKIAFLPFGY LVDQWRWGVF SGRTPPSRYN FDWWYLRTKY QGICPPVTRN ETHFDAGAKF HVPNVTPYIR YFVSFVLQFQ FHEALCKEAG YEGPLHQCDI YRSTKAGAKL RKVLQAGSSR PWQEVLKDMV GLDALDAQPL LKYFQPVTQW LQEQNQQNGE VLGWPEYQWH PPLPDNYPEG IDLVTDEAEA SKFVEEYDRT SQVVWNEYAE ANWNYNTNIT TETSKILLQK NMQIANHTLK YGTQARKFDV NQLQNTTIKR IIKKVQDLER AALPAQELEE YNKILLDMET TYSVATVCHP NGSCLQLEPD LTNVMATSRK YEDLLWAWEG WRDKAGRAIL QFYPKYVELI NQAARLNGYV DAGDSWRSMY ETPSLEQDLE RLFQELQPLY LNLHAYVRRA LHRHYGAQHI NLEGPIPAHL LGNMWAQTWS NIYDLVVPFP SAPSMDTTEA MLKQGWTPRR MFKEADDFFT SLGLLPVPPE FWNKSMLEKP TDGREVVCHA SAWDFYNGKD FRIKQCTTVN LEDLVVAHHE MGHIQYFMQY KDLPVALREG ANPGFHEAIG DVLALSVSTP KHLHSLNLLS SEGGSDEHDI NFLMKMALDK IAFIPFSYLV DQWRWRVFDG SITKENYNQE WWSLRLKYQG LCPPVPRTQG DFDPGAKFHI PSSVPYIRYF VSFIIQFQFH EALCQAAGHT GPLHKCDIYQ SKEAGQRLAT AMKLGFSRPW PEAMQLITGQ PNMSASAMLS YFKPLLDWLR TENELHGEKL GWPQYNWTPN SARSEGPLPD SGRVSFLGLD LDAQQAR

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Leu 30

Product Type Name :

Recombinant Protein

UniProt No. :

P12821

Internal Search Terms :

,ATGP3944-10ug,ATGP3944-20ug,ATGP3944-50ug,ATGP3944-100ug,ATGP3944-250ug,ATGP3944-500ug,ATGP3944-1mg,ATGP3944-10,ATGP3944-20,ATGP3944-50,ATGP3944-100,ATGP3944-250,ATGP3944-500,ATGP3944-1

more info or order :

NKMAX product webpage