Recombinant human PON1 protein | NKMAX ATGP3936 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PON1 protein

catalog :

ATGP3936

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PON1 protein

Domain :

16-355aa

Catalog number (SKU) :

ATGP3936

NCBI :

NP_000437.3

Description :

PON1, also known as A esterase1, is a member of the paraoxonase family. It is an enzyme that hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). This protein is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis. PON1 shows a variety of atheroprotective properties by metabolizing inflammatory lipid peroxides. It has evolved to be a highly promiscuous enzyme capable of hydrolysing a wide variety of substrates such as lactones, cyclic carbonates, organophosphorus pesticides and nerve gases. Recombinant human PON1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Alternative Names :

Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, paraoxonase 1, ESA, MVCD5, PON

Concentration :

0.25mg/ml (determined by Absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Banhela N., et al. (2020) Syst Rev. 9:109.;; Soliman AM., et al. (2020) Eur J Med Chem. 197:112333.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

39.0kDa (346aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.

Amino Acid Sequence :

LFRNHQSSYQ TRLNALREVQ PVELPNCNLV KGIETGSEDL EILPNGLAFI SSGLKYPGIK SFNPNSPGKI LLMDLNEEDP TVLELGITGS KFDVSSFNPH GISTFTDEDN AMYLLVVNHP DAKSTVELFK FQEEEKSLLH LKTIRHKLLP NLNDIVAVGP EHFYGTNDHY FLDPYLQSWE MYLGLAWSYV VYYSPSEVRV VAEGFDFANG INISPDGKYV YIAELLAHKI HVYEKHANWT LTPLKSLDFN TLVDNISVDP ETGDLWVGCH PNGMKIFFYD SENPPASEVL RIQNILTEEP KVTQVYAENG TVLQGSTVAS VYKGKLLIGT VFHKALYCEL

Tag :

His-Tag

Expression System :

HEK293

Predicted nterm :

Leu 16

Product Type Name :

Recombinant Protein

UniProt No. :

P27169

Internal Search Terms :

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more info or order :

NKMAX product webpage