Recombinant firefly Luciferase protein | NKMAX ATGP3928 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant firefly Luciferase protein

catalog :

ATGP3928

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant firefly Luciferase protein

Domain :

1-550aa

Catalog number (SKU) :

ATGP3928

NCBI :

AB261982

Description :

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Luciferin 4-monooxygenase, LuC

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Moore S A., et al. (1995) J Mol Biol. 249(1):195-214;; Gould S J., et al. (1988) Anal Biochem. 175(1):5-13.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is 1X10^10 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at 25C

Applications :

SDS-PAGE,Enzyme Activity

Antigen Species :

Firefly

Gene Category :

Enzymes & Proteases

Molecular Weight :

63 kDa (571aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is 1X10^10 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at 25C

Amino Acid Sequence :

MEDAKNIKK GPAPFYPLED GTAGEQLHKA MKRYALVPGT IAFTDAHIEV NITYAEYFEM SVRLAEAMKR YGLNTNHRIV VCSENSLQFF MPVLGALFIG VAVAPANDIY NERELLNSMN ISQPTVVFVS KKGLQKILNV QKKLPIIQKI IIMDSKTDYQ GFQSMYTFVT SHLPPGFNEY DFVPESFDRD KTIALIMNSS GSTGLPKGVA LPHRTACVRF SHARDPIFGN QIIPDTAILS VVPFHHGFGM FTTLGYLICG FRVVLMYRFE EELFLRSLQD YKIQSALLVP TLFSFFAKST LIDKYDLSNL HEIASGGAPL SKEVGEAVAK RFHLPGIRQG YGLTETTSAI LITPEGDDKP GAVGKVVPFF EAKVVDLDTG KTLGVNQRGE LCVRGPMIMS GYVNNPEATN ALIDKDGWLH SGDLAYWDED EHFFIVDRLK SLIKYKGYQV APAELESILL QHPNIFDAGV AGLPDDDAGE LPAAVVVLEH GKTMTEKEIV DYVASQVTTA KKLRGGVVFV DEVPKGLTGK LDARKIREIL IKAKKGGKSK L

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

A3KBZ5

Internal Search Terms :

Luciferase,ATGP3928-10ug,ATGP3928-20ug,ATGP3928-50ug,ATGP3928-100ug,ATGP3928-250ug,ATGP3928-500ug,ATGP3928-1mg,ATGP3928-10,ATGP3928-20,ATGP3928-50,ATGP3928-100,ATGP3928-250,ATGP3928-500,ATGP3928-1

more info or order :

NKMAX product webpage