protein

Recombinant human Kallikrein 3/PSA protein

ATGP3926

10 ug

160 USD

Recombinant human Kallikrein 3/PSA protein

18-261aa

ATGP3926

NP_001639

Kallikrein 3, as known as prostate specific antigen (PSA), is a subgroup of serine proteases, enzymes capable of cleaving peptide bonds in proteins. This protein is expressed in the prostate and is thought to be responsible for regulating semen liquefaction through hydrolysis of seminogelin. It is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia. Recombinant human Kallikrein 3 protein, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, APS, hK3, KLK2A1, kallikrein related peptidase 3, prostate-specific antigen isoform 1

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Hochmeister MN. et al. (1999) J Forensic Sci. J. 44:1057-1060. ;;Jain, S. et al. (2002) Postgrad. Med. J. 78:646-650.

> 95% by SDS-PAGE

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme cleaves 1pmole of Succinyl-Arg-Pro-Tyr-p-Nitroanilide to Succinyl-Arg-Pro-Tyr and p-Nitroanilide per minute at pH7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Neuroscience

27.6 kDa (250aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme cleaves 1pmole of Succinyl-Arg-Pro-Tyr-p-Nitroanilide to Succinyl-Arg-Pro-Tyr and p-Nitroanilide per minute at pH7.5 at 37C.

APLILSRIVG GWECEKHSQP WQVLVASRGR AVCGGVLVHP QWVLTAAHCI RNKSVILLGR HSLFHPEDTG QVFQVSHSFP HPLYDMSLLK NRFLRPGDDS SHDLMLLRLS EPAELTDAVK VMDLPTQEPA LGTTCYASGW GSIEPEEFLT PKKLQCVDLH VISNDVCAQV HPQKVTKFML CAGRWTGGKS TCSGDSGGPL VCNGVLQGIT SWGSEPCALP ERPSLYTKVV HYRKWIKDTI VANP

His-Tag

HEK293

Ala

Recombinant Protein

P07288

Seminin, Semenogelase, PSA, Prostate-specific antigen isoform 1, Prostate-specific antigen, Prostate specific antigen isoform 1, Prostate specific antigen, P-30 antigen, P30 antigen, KLK3, KLK2A1, Kallikrein-3, Kallikrein3, Kallikrein related peptidase 3, Kallikrein 3, hK3, Gamma-seminoprotein, APS, Gamma seminoprotein, ATGP3926-10ug, ATGP3926-20ug, ATGP3926-50ug, ATGP3926-100ug, ATGP3926-250ug, ATGP3926-500ug, ATGP3926-1mg, ATGP3926-010, ATGP3926-020, ATGP3926-050, ATGP3926-100, ATGP3926-250, ATGP3926-500, ATGP3926-01M