Recombinant human CTLA-4 protein | NKMAX ATGP3918 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CTLA-4 protein

catalog :

ATGP3918

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CTLA-4 protein

Domain :

36-161aa

Catalog number (SKU) :

ATGP3918

NCBI :

NP_005205.2

Description :

CTLA-4, also known as cytotoxic T-lymphocyte protein 4, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells. It also is a protein receptor that functions as an immune checkpoint and downregulates immune responses. This protein is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation a phenomenon which is particularly notable in cancers. It may also function via modulation of cell motility and signaling through PI3 kinase. It has been implicated in several autoimmune diseases, such as systemic lupus erythematosus. Recombinant human CTLA-4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, CELIAC3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, IDDM12, CD, GSE

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Dai F., et al. (2015) Braz J Med Biol Res. 48:629-636. ;;Ward FJ., et al. (2013) Eur J Immunol. 43:1274-1285.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

The activity is determined by the IL-2 ELISA in a using Jurkat human acute T cell leukemia cells. The ED50 range ≤150ng/ml with Human B7-1/CD80.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Additional Comment Only :

ATGP3334 has been replaced with a catalog number ATGP3918.

Molecular Weight :

14.6 kDa (135aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human CTLA-4 inhibits Human B7-1/CD80 induced IL-2 secretion of the Jurkat human acute T cell leukemia cells. The ED50 range ≤150 ng/ml.

Amino Acid Sequence :

KAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSD

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P16410

Internal Search Terms :

Cytotoxic T lymphocyte protein 4 isoform CTLA4-TM precursor, Cytotoxic T-lymphocyte-associated antigen 4, CTLA 4, CTLA4, CTLA-4, CD 152, CD152, CELIAC3, CELIAC 3, Celiac disease 3, Insulin-dependent diabetes mellitus 12, Insulin dependent diabetes mellitus 12, IDDM 12, IDDM12, CD, GSE, ATGP3918-10ug, ATGP3918-20ug, ATGP3918-50ug, ATGP3918-100ug, ATGP3918-250ug, ATGP3918-500ug, ATGP3918-1mg, ATGP3918-010, ATGP3918-020, ATGP3918-050, ATGP3918-100, ATGP3918-250, ATGP3918-500, ATGP3918-01M, ATGP3334

more info or order :

NKMAX product webpage