Recombinant human BACE-1 protein | NKMAX ATGP3917 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human BACE-1 protein

catalog :

ATGP3917

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human BACE-1 protein

Domain :

22-457aa

Catalog number (SKU) :

ATGP3917

NCBI :

NP_036236

Description :

BACE-1, also known as beta-secretase 1 isoform A, is a member of aspartic protease and an integral membrane protein. It is involved in the proteolytic processing of the amyloid precursor protein (APP). This protein cleaves at the N-terminus of the A-beta peptide sequence of APP, and then leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has been implicated in the onset and/or progression of Alzheimer's disease. It is also distantly related to the pathogenic aspartic-acid protease plasmepsin, which is a potential target for future anti-malarial drugs. Recombinant human BACE-1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

BACE1, Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE, KIAA1149, HSPC104

Concentration :

0.5mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Andrew RJ., et al. (2013) Proc Natl Acad Sci U S A. 114:E9665-E9674.;; Brendel M., et al. (2018) Theranostics. 8:4957-4968.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 5pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

49.2 kDa (442aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 5 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.

Amino Acid Sequence :

TQHGIRLPLR SGLGGAPLGL RLPRETDEEP EEPGRRGSFV EMVDNLRGKS GQGYYVEMTV GSPPQTLNIL VDTGSSNFAV GAAPHPFLHR YYQRQLSSTY RDLRKGVYVP YTQGKWEGEL GTDLVSIPHG PNVTVRANIA AITESDKFFI NGSNWEGILG LAYAEIARPD DSLEPFFDSL VKQTHVPNLF SLQLCGAGFP LNQSEVLASV GGSMIIGGID HSLYTGSLWY TPIRREWYYE VIIVRVEING QDLKMDCKEY NYDKSIVDSG TTNLRLPKKV FEAAVKSIKA ASSTEKFPDG FWLGEQLVCW QAGTTPWNIF PVISLYLMGE VTNQSFRITI LPQQYLRPVE DVATSQDDCY KFAISQSSTG TVMGAVIMEG FYVVFDRARK RIGFAVSACH VHDEFRTAAV EGPFVTLDME DCGYNIPQTD ESTLMT

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Thr22

Product Type Name :

Recombinant Protein

UniProt No. :

P56817

Internal Search Terms :

BACE1,ATGP3917-10ug,ATGP3917-20ug,ATGP3917-50ug,ATGP3917-100ug,ATGP3917-250ug,ATGP3917-500ug,ATGP3917-1mg,ATGP3917-10,ATGP3917-20,ATGP3917-50,ATGP3917-100,ATGP3917-250,ATGP3917-500,ATGP3917-1

more info or order :

NKMAX product webpage