protein

Recombinant mouse TRANCE/RANKL/TNFSF11 protein

ATGP3915

10 ug

160 USD

Recombinant mouse TRANCE/RANKL/TNFSF11 protein

158-316aa

ATGP3915

NP_035743

Tnfsf11, also known as tumor necrosis factor ligand superfamily member 11, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. Tnfsf11 also has a function in the immune system, where it is expressed by T helper cells and is thought to be involved in dendritic cell maturation. Tnfsf11 is important in bone metabolism. This natural and necessary surface-bound molecule (also known as CD254) found on osteoblasts serves to activate osteoclasts, which are the cells involved in bone resorption. Recombinant Mouse Tnfsf11 protein was expressed in E. coli and purified by using conventional chromatography.

Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

1mg/ml (determined by Bradford assay)

Liquid In. 20mM Tris-HCl buffer (pH8.5) containing 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lam J., et al. (2001). J. Clin. Invest. 108(7):971-9.;;Ito S., et al. (2002). J. Biol. Chem. 277(8):6631-6.

> 90% by SDS-PAGE

Measured in a osteoclast differentiation assay using RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2ng/ml.

SDS-PAGE, Bioactivity

Mouse

Hormones & Cytokines

17.9 kDa (160aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Mouse TRANCE/RANK L/TNFSF11 stimulates osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤2 ng/ml.

MKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID

Non-Tagged

E.coli

Recombinant Protein

O35235

TRANCE, RANK L, TNFSF11,ATGP3915-10ug,ATGP3915-20ug,ATGP3915-50ug,ATGP3915-100ug,ATGP3915-250ug,ATGP3915-500ug,ATGP3915-1mg,ATGP3915-10,ATGP3915-20,ATGP3915-50,ATGP3915-100,ATGP3915-250,ATGP3915-500,ATGP3915-1

Park KH, et al. Zinc inhibits osteoclast differentiation by suppression of Ca2+-Calcineurin-NFATc1 signaling pathway. (Cell Commun Signal. 2013){https://pubmed.ncbi.nlm.nih.gov/24088289/}