Recombinant mouse Granzyme B protein | NKMAX ATGP3913 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Granzyme B protein

catalog :

ATGP3913

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Granzyme B protein

Domain :

19-247aa

Catalog number (SKU) :

ATGP3913

NCBI :

NP_038570

Description :

Granzyme B, also known as Gzmb, is a member of the granzyme family of cell death-inducing serine proteases specially expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. It plays crucial role for target cell lysis in cell-mediated immune responses. This protein is linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. It cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Recombinant mouse Granzyme B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Gzmb, CTLA-1, Cytotoxic cell protease 1, CCP1, Fragmentin-2, Ctla-1, Ctla1, AI553453, CCP-1/C11, CCP1, GZB

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

D' Eliseo D., et al. (2016) J Exp Clin Cancer Res. 35:24.;; Rabani M., et al. (2018) Clin Immunol. 188:45-51.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 9,000pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Apoptosis

Molecular Weight :

26.3 kDa (235aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Amino Acid Sequence :

GEIIGGHEVK PHSRPYMALL SIKDQQPEAI CGGFLIREDF VLTAAHCEGS IINVTLGAHN IKEQEKTQQV IPMVKCIPHP DYNPKTFSND IMLLKLKSKA KRTRAVRPLN LPRRNVNVKP GDVCYVAGWG RMAPMGKYSN TLQEVELTVQ KDRECESYFK NRYNKTNQIC AGDPKTKRAS FRGDSGGPLV CKKVAAGIVS YGYKDGSPPR AFTKVSSFLS WIKKTMKSS

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Gly19

Product Type Name :

Recombinant Protein

UniProt No. :

P04187

Internal Search Terms :

Granzyme B,ATGP3913-10ug,ATGP3913-20ug,ATGP3913-50ug,ATGP3913-100ug,ATGP3913-250ug,ATGP3913-500ug,ATGP3913-1mg,ATGP3913-10,ATGP3913-20,ATGP3913-50,ATGP3913-100,ATGP3913-250,ATGP3913-500,ATGP3913-1

more info or order :

NKMAX product webpage