Recombinant human Neprilysin/CD10 protein | NKMAX ATGP3907 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Neprilysin/CD10 protein

catalog :

ATGP3907

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Neprilysin/CD10 protein

Domain :

52-750aa

Catalog number (SKU) :

ATGP3907

NCBI :

NP_000893

Description :

Neprilysin, also known as MME, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. It degrades the amyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. It is expressed in a wide variety of tissues and is particularly abundant in kidney. It is also a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). It is of use in hematological diagnosis since it is expressed by early B, pro-B and pre-B lymphocytes, and by lymph node germinal centers. Recombinant human neprilysin, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

MME, Neprilysin, CALLA, CD10, CMT2T, NEP, SCA43, SFE, Atriopeptidase, Common acute lymphocyticleukemia antigen, Enkephalinase, Neutral endopeptidase, Skin fibroblast elastase, Membrane metalloendopeptidase, EPN

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 0.1mM PMSF, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Auer-Grumbach M., et al. (2016) Am J Hum Genet. 99:607-623.;;Hama E., et al. (2005) Med Hypotheses. 65:498-500.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 5,000pmol/min/ug in which one unit will convert 1.0pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

80.9 kDa (708aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 5,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-SEVNLDAEFRK(Dnp)RR-NH2 to MCA- Pro-Leu-OH per minute at pH 8.8 at 25C.

Amino Acid Sequence :

YDDGICK SSDCIKSAAR LIQNMDATTE PCTDFFKYAC GGWLKRNVIPETSSRYGNFDILRDELEVVL KDVLQEPKTE DIVAVQKAKA LYRSCINESA IDSRGGEPLLKLLPDIYGWPVATENWEQKY GASWTAEKAI AQLNSKYGKK VLINLFVGTD DKNSVNHVIHIDQPRLGLPSRDYYECTGIY KEACTAYVDF MISVARLIRQ EERLPIDENQ LALEMNKVMELEKEIANATAKPEDRNDPML LYNKMTLAQI QNNFSLEING KPFSWLNFTN EIMSTVNISITNEEDVVVYAPEYLTKLKPI LTKYSARDLQ NLMSWRFIMD LVSSLSRTYK ESRNAFRKALYGTTSETATWRRCANYVNGN MENAVGRLYV EAAFAGESKH VVEDLIAQIR EVFIQTLDDLTWMDAETKKRAEEKALAIKE RIGYPDDIVS NDNKLNNEYL ELNYKEDEYF ENIIQNLKFSQSKQLKKLREKVDKDEWISG AAVVNAFYSS GRNQIVFPAG ILQPPFFSAQ QSNSLNYGGIGMVIGHEITHGFDDNGRNFN KDGDLVDWWT QQSASNFKEQ SQCMVYQYGN FSWDLAGGQHLNGINTLGENIADNGGLGQA YRAYQNYIKK NGEEKLLPGL DLNHKQLFFL NFAQVWCGTYRPEYAVNSIKTDVHSPGNFR IIGTLQNSAE FSEAFHCRKN SYMNPEKKCR VW

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P08473

Internal Search Terms :

Neprilysin,ATGP3907-10ug,ATGP3907-20ug,ATGP3907-50ug,ATGP3907-100ug,ATGP3907-250ug,ATGP3907-500ug,ATGP3907-1mg,ATGP3907-10,ATGP3907-20,ATGP3907-50,ATGP3907-100,ATGP3907-250,ATGP3907-500,ATGP3907-1

more info or order :

NKMAX product webpage