Recombinant mouse Carboxyl Ester Lipase/CEL protein | NKMAX ATGP3905 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Carboxyl Ester Lipase/CEL protein

catalog :

ATGP3905

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Carboxyl Ester Lipase/CEL protein

Domain :

21-599aa

Catalog number (SKU) :

ATGP3905

NCBI :

NP_034015

Description :

CEL, also known as bile salt-activated lipase, is one of the Type-B carboxyl esterase/lipase family of enzymes. It is an enzyme produced by the adult pancreas and aids in the digestion of fats. Also, it catalyzes fat and vitamin absorption and acts in concert with pancreatic lipase and colipase for the complete digestion of dietary triglycerides. This protein is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract. Recombinant mouse CEL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Cel, 1810036E18Rik, BAL, BSSL, Carboxyl ester lipase, Cholesterol esterase, Pancreaticlysophospholipase, Sterol esterase, Lip1, Bile salt-activated lipase, Bile salt-stimulated lipase

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Xiao X., et al. (2016) J Biol Chem. 291:23224-23236.;; Shindo K., et al. (2017) Oncotarget. 8:50824-50831.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 100,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Cell Cycle

Molecular Weight :

64.5 kDa (585aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 100,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 umoleof p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C

Amino Acid Sequence :

AKLGAVYTEG GFVEGVNKKL SLLGGDSVDI FKGIPFATAK TLENPQRHPG WQGTLKATNF KKRCLQATIT QDNTYGQEDC LYLNIWVPQG RKQVSHNLPV MVWIYGGAFL MGSGQGANFL KNYLYDGEEI ATRGNVIVVT FNYRVGPLGF LSTGDANLPG NFGLRDQHMA IAWVKRNIAA FGGDPDNITI FGESAGAASV SLQTLSPYNK GLIRRAISQS GMALSPWAIQ KNPLFWAKTI AKKVGCPTED TGKMAACLKI TDPRALTLAY KLPVKKQEYP VVHYLAFIPV IDGDFIPDDP INLYNNTADI DYIAGINNMD GHLFATIDVP AVDKTKQTVT EEDFYRLVSG HTVAKGLKGA QATFDIYTES WAQDPSQENM KKTVVAFETD VLFLIPTEIA LAQHKAHAKS AKTYSYLFSH PSRMPIYPKW MGADHADDLQ YVFGKPFATP LGYRPQDRAV SKAMIAYWTN FARSGDPNMG NSPVPTHWYP YTLENGNYLD ITKTITSASM KEHLREKFLK FWAVTFEVLP TVTGDQDTLT PPEDDSEVAP DPPSDDSQVV PVPPTDDSVE AQMPATIGF

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala21

Product Type Name :

Recombinant Protein

UniProt No. :

Q64285

Internal Search Terms :

Carboxyl Ester Lipase, CEL,ATGP3905-10ug,ATGP3905-20ug,ATGP3905-50ug,ATGP3905-100ug,ATGP3905-250ug,ATGP3905-500ug,ATGP3905-1mg,ATGP3905-10,ATGP3905-20,ATGP3905-50,ATGP3905-100,ATGP3905-250,ATGP3905-500,ATGP3905-1

more info or order :

NKMAX product webpage