Recombinant human Sulfatase Modifying Factor 1/SUMF1 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Sulfatase Modifying Factor 1/SUMF1 protein

catalog :

ATGP3893

quantity :

10 ug

price :

140 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Sulfatase Modifying Factor 1/SUMF1 protein

Domain :

34-374aa

Catalog number (SKU) :

ATGP3893

NCBI :

NP_877437.2

Description :

SUMF1, also known as sulfatase-modifying factor 1 isoform 1, is a Ca2+-binging member of the sulfatase-modifying factor family. This protein as soluble ER lumenal glycoprotein converts inactive sulfatases into an active form by transforming a catalytic site cysteine into a formylglycine residue. In the ER, it can exist as either a monomer, or a disulfide-linked homodimer or a heterodimer with SUMF2. The genetic defect of FGly formation caused by mutations in the SUMF1 gene results in inactive FGE, and subsequently multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant Human SUMF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

SUMF1, AAPA3037, FGE, UNQ3037, Formylglycine-generating enzyme, C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Preusser-Kunze A., et al, (2005) J. Biol. Chem. 280:14900-14910.;;Zito E., et al, (2005) EMBO Rep. 6:655-660.

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Cytoskeleton

Molecular Weight :

38.1 kDa (347aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence :

SQEAGTGAGA GSLAGSCGCG TPQRPGAHGS SAAAHRYSRE ANAPGPVPGE RQLAHSKMVP IPAGVFTMGT DDPQIKQDGE APARRVTIDA FYMDAYEVSN TEFEKFVNST GYLTEAEKFG DSFVFEGMLS EQVKTNIQQA VAAAPWWLPV KGANWRHPEG PDSTILHRPD HPVLHVSWND AVAYCTWAGK RLPTEAEWEY SCRGGLHNRL FPWGNKLQPK GQHYANIWQG EFPVTNTGED GFQGTAPVDA FPPNGYGLYN IVGNAWEWTS DWWTVHHSVE ETLNPKGPPS GKDRVKKGGS YMCHRSYCYR YRCAARSQNT PDSSASNLGF RCAADRLPTM D

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ser34

Product Type Name :

Recombinant Protein

UniProt No. :

Q8NBK3

Internal Search Terms :

UNQ3037, SUMF1, SUMF 1, Sulfatase-modifying factor1, Sulfatase-modifying factor 1, Sulfatase modifying factor1, Sulfatase modifying factor 1, Formylglycine-generating enzyme, Formylglycine generating enzyme, FGE, C-alpha-formylglycine-generating enzyme1, C-alpha-formylglycine-generating enzyme 1, C alpha formylglycine generating enzyme1, C alpha formylglycine generating enzyme 1, AAPA3037, ATGP3893-10ug, ATGP3893-20ug, ATGP3893-50ug, ATGP3893-100ug, ATGP3893-250ug, ATGP3893-500ug, ATGP3893-1mg, ATGP3893-010, ATGP3893-020, ATGP3893-050, ATGP3893-100, ATGP3893-250, ATGP3893-500, ATGP3893-01M

more info or order :

NKMAX product webpage