Recombinant human B3GAT1 protein | NKMAX ATGP3892 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human B3GAT1 protein

catalog :

ATGP3892

quantity :

10 ug

price :

140 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human B3GAT1 protein

Domain :

28-334aa

Catalog number (SKU) :

ATGP3892

NCBI :

NP_473366

Description :

B3GAT1, also known as galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, is a key enzyme during the biosynthesis of the carbohydrate epitope HNK-1. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This protein is expressed as a carbohydrate epitope that contains a sulfoglucuronyl residue in several adhesion molecules of the nervous system. Also, the enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. This protein functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1. Recombinant Human B3GAT1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Terayama K., et al, (1997) Proc. Natl. Acad. Sci. USA 94:6093-6098.;;Kakuda, S., et al, (2005) Glycobiology 2:203-210.

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

62.3 kDa (549aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence :

TLAPLLA VHKDEGSDPR RETPPGADPR EYCTSDRDIV EVVRTEYVYT RPPPWSDTLP TIHVVTPTYS RPVQKAELTR MANTLLHVPN LHWLVVEDAP RRTPLTARLL RDTGLNYTHL HVETPRNYKL RGDARDPRIP RGTMQRNLAL RWLRETFPRN SSQPGVVYFA DDDNTYSLEL FEEMRSTRRV SVWPVAFVGG LRYEAPRVNG AGKVVGWKTV FDPHRPFAID MAGFAVNLRL ILQRSQAYFK LRGVKGGYQE SSLLRELVTL NDLEPKAANC TKILVWHTRT EKPVLVNEGK KGFTDPSVEI

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

Q9P2W7

Internal Search Terms :

B3GAT1, ATGP3892-10ug,ATGP3892-20ug,ATGP3892-50ug,ATGP3892-100ug,ATGP3892-250ug,ATGP3892-500ug,ATGP3892-1mg, ATGP3892-10,ATGP3892-20,ATGP3892-50,ATGP3892-100,ATGP3892-250,ATGP3892-500,ATGP3892-1,ATGP3892-10ug,ATGP3892-20ug,ATGP3892-50ug,ATGP3892-100ug,ATGP3892-250ug,ATGP3892-500ug,ATGP3892-1mg,ATGP3892-10,ATGP3892-20,ATGP3892-50,ATGP3892-100,ATGP3892-250,ATGP3892-500,ATGP3892-1

more info or order :

NKMAX product webpage