protein

Recombinant mouse SPARC-like 1/SPARCL1 protein

ATGP3891

10 ug

120 USD

Recombinant mouse SPARC-like 1/SPARCL1 protein

17-650aa

ATGP3891

NP_034227

SPARC1, also known as SPARC-like protein 1, is a secreted protein with high structural similarity to SPARC. This protein as an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. It inhibits adhesion and spreading on a variety of substrates and is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. Also, it binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Recombinant Mouse SPARCL1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Sparcl1, Ecm2, hevin, mast9, Sc1, SPARC-like protein 1, Extracellular matrix protein 2, Matrix glycoprotein Sc1

0.25mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991-996.;;Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735-748.

> 90% by SDS-PAGE

SDS-PAGE

Mouse

Cancer

71.7 kDa (642aa)

< 1 EU per 1ug of protein (determined by LAL method)

IPTSTRFLSD HSNPTTATLV TPEDATVPIA GVEATADIEN HPSDKAEKPS ALNSEEETHE QSTEQDKTYS FEVDLKDEED GDGDLSVDPT EGTLTLDLQE GTSEPQQKSL PENGDFPATV STSYVDPNQR ANITKGKESQ EQPVSDSHQQ PNESSKQTQD LKAEESQTQD PDIPNEEEEE EEDEEEEEEE EPEDIGAPSD NQEEGKEPLE EQPTSKWEGN REQSDDTLEE SSQPTQISKT EKHQSEQGNQ GQESDSEAEG EDKAAGSKEH IPHTEQQDQE GKAGLEAIGN QKDTDEKAVS TEPTDAAVVP RSHGGAGDNG GGDDSKHGAG DDYFIPSQEF LEAERMHSLS YYLKYGGGEE TTTGESENRR EAADNQEAKK AESSPNAEPS DEGNSREHSA GSCTNFQCKR GHICKTDPQG KPHCVCQDPE TCPPAKILDQ ACGTDNQTYA SSCHLFATKC RLEGTKKGHQ LQLDYFGACK SIPACTDFEV AQFPLRMRDW LKNILMQLYE PNPKHGGYLN EKQRSKVKKI YLDEKRLLAG HPIELLLRD FKKNYHMYVY PVHWQFNELD QHPADRILTH SELAPLRASL VPMEHCITRF FEECDPNKDK HITLKEWGHC FGIKEEDIDE NLLF

His-Tag

Baculovirus

Ile17

Recombinant Protein

P70663

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Hattori T, et al. Astroglial CD38 regulates social memory and synapse formation through SPARCL1 in the medial prefrontal cortex. (bioRxiv. 2021){https://doi.org/10.1101/2021.12.23.474051};;Hattori T, et al. Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory. (EMBO J. 2023){https://pubmed.ncbi.nlm.nih.gov/37357972/}