protein

Recombinant mouse ALT2/GPT2 protein

ATGP3881

50 ug

480 USD

Recombinant mouse ALT2/GPT2 protein

1-522aa

ATGP3881

NP_776291

ALT2/GPT2, also known as alanine aminotransferase, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. Recombinant mouse ALT2/GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alanine aminotransferase 2, ALT2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamate pyruvate transaminase 2

1mg/ml (determined by absorbance at 280nm)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 2mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Sohocki M.M. et al. (1997) Genomics 40(2):247-52.;; Matthews C.C. et al. (2003) Brain Res. 978(1-2): 59-64.

> 85% by SDS-PAGE

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.

SDS-PAGE, Enzyme Activity

Mouse

Metabolism

60.1 kDa (542aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 50units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.

QRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS

His-Tag

E.coli

Recombinant Protein

Q8BGT5

Alanine aminotransferase2, Alanine aminotransferase 2, ALT 2, ALT2, ALT-2, Glutamate pyruvate transaminase 2, Glutamic alanine transaminase 2, Glutamic pyruvic transaminase 2, Glutamic-alanine transaminase 2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, GPT 2, GPT2, GPT-2, ATGP3881-10ug, ATGP3881-20ug, ATGP3881-50ug, ATGP3881-100ug, ATGP3881-250ug, ATGP3881-500ug, ATGP3881-1mg, ATGP3881-010, ATGP3881-020, ATGP3881-050, ATGP3881-100, ATGP3881-250, ATGP3881-500, ATGP3881-01M