product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant mouse Bleomycin Hydrolase/BLMH protein
catalog :
ATGP3880
quantity :
10 ug
price :
185 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant mouse Bleomycin Hydrolase/BLMH protein
Domain :
1-455aa
Catalog number (SKU) :
ATGP3880
NCBI :
NP_848760
Description :
BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug Blm. It catalyzes the inactivation of the antitumor drug Blm (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant mouse BLMH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Alternative Names :
Bleomycin hydrolase, BH, BLM hydrolase, BMH
Concentration :
0.25mg/ml (determined by absorbance at 280nm)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 30% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Montoya SE.,et al. (2007) Neuroscience. 146(3):890-900.;; Suszynska-Zajczyk J.,et al. (2014) Mol Genet Metab.112(4):339-46.
Purity :
> 90% by SDS-PAGE
Bioactivity Comment Only :
Specific activity is > 1,500pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.
Applications :
SDS-PAGE, Enzyme Activity
Antigen Species :
Mouse
Gene Category :
Neuroscience
Molecular Weight :
54.9 kDa (478aa) confirmed by MALDI-TOF
Bioactivity Comment and Pics :
Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.
Amino Acid Sequence :
MNNAGLN SEKVSALIQK LNSDPQFVLA QNVGTTHDLL DICLRRATVQ GAQHVFQHVV PQEGKPVTNQ KSSGRCWIFS CLNVMRLPFM KKFNIEEFEF SQSYLFFWDK VERCYFFLNA FVDTAQKKEP EDGRLVQYLL MNPTNDGGQW DMLVNIVEKY GVVPKKCFPE SHTTEATRRM NDILNHKMRE FCIRLRNLVH SGATKGEISS TQDAMMEEIF RVVCICLGNP PETFTWEYRD KDKNYHKIGP ITPLQFYKEH VKPLFNMEDK ICFVNDPRPQ HKYNKLYTVD YLSNMVGGRK TLYNNQPIDF LKKMVAASIK DGEAVWFGCD VGKHFNGKLG LSDMNVYDHE LVFGVSLKNM NKAERLAFGE SLMTHAMTFT AVSEKDNQEG TFVKWRVENS WGEDHGHKGY LCMTDEWFSE YVYEVVVDKK HVPEEVLAVL EQEPIVLPAW DPMGALAE
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q8R016
Internal Search Terms :
AI035728, BH, Bleomycin hydrolase, Blm hydrolase, BLMH, Bmh, EC 3.4.22.40, ATGP3880-10ug, ATGP3880-20ug, ATGP3880-50ug, ATGP3880-100ug, ATGP3880-250ug, ATGP3880-500ug, ATGP3880-1mg, ATGP3880-010, ATGP3880-020, ATGP3880-050, ATGP3880-100, ATGP3880-250, ATGP3880-500, ATGP3880-01M
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.