protein

Recombinant human NQO1 protein

ATGP3876

10 ug

176 USD

Recombinant human NQO1 protein

1-274aa

ATGP3876

NP_000894

NQO1 is a member of the NAD (P) H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Recombinant human NQO1, was expressed in E. coli and purified by using conventional chromatography techniques.

NAD(P)H quinone dehydrogenase 1, NMOR1, DIA4, Diaphorase, NADH/NADPH, Cytochrome b-5 reductase, NAD(P)H dehydrogenase, Quinone 1, DHQU, QR1, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Jaiswal AK., et al. (1988) J Biol Chem. 263(27):13572-8.;;Traver RD., et al. (1992) Cancer Res. 52(4):797-802.

> 95% by SDS-PAGE

Specific activity is > 1,000pmol/min/ug and is defined as the amount of enzyme that converts 1pmole of resazurin to resorufin per minute at pH 7.5 at 25C.

SDS-PAGE, Enzyme Activity

Human

Redox Proteins

33.0 kDa (294aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 1,000 pmol/min/ug and is defined as the amount of enzyme that converts 1 pmole of resazurin to resorufin per minute at pH 7.5 at 25C.

MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK

His-Tag

E.coli

Recombinant Protein

P15559

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