protein

Recombinant human eIF-3 beta/EIF3I protein

ATGP3875

10 ug

80 USD

Recombinant human eIF-3 beta/EIF3I protein

1-325aa

ATGP3875

NP_003748.1

EIF3I, also known as eukaryotic translation initiation factor 3 subunit I, is a multiprotein complex that functions during the initiation phase of eukaryotic translation. This protein is one out of 13 subunits of the mammalian EIF3 complex which associated with the 40S ribosome and facilitates the formation of 43S pre-initiation complex. This complex stimulates nearly all steps of translation initiation. These factors also appear to participate in other phases of translation, such as recycling, where it promotes the splitting of post-termination ribosomes. Also, it is shown to act as a signaling transducer by bind to and activating AKT1 through preventing PP2A-induced AKT1 dephosphorylation in tumor cells. Recombinant Human EIF3I, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

C3orf68, eIF3-beta, eIF-3-beta, eIF3i, eIF3-p36, EIF3S2, Eukaryotic translation initiation factor 3 subunit 2, Eukaryotic translation initiation factor 3 subunit 2 beta 36kDa, Eukaryotic translation initiation factor 3 subunit I, PRO2242, TGF-beta receptor-interacting protein 1, TRIP1, TRIP-1

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 40% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Mayeur GL., et al, (2003) Eur. J. Biochem. 270:4133-4139.;;Pan W., et al, (2017) Vet. Microbiol. 212:59-66.

> 90% by SDS-PAGE

SDS-PAGE

Human

Epigenomics (Transcription & Translation)

37.3 kDa (331aa)

< 1 EU per 1ug of protein (determined by LAL method)

MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG FDFGGNIIMF STDKQMGYQC FVSFFDLRDP SQIDNNEPYM KIPCNDSKIT SAVWGPLGEC IIAGHESGEL NQYSAKSGEV LVNVKEHSRQ INDIQLSRDM TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPN YDHVVLGGGQ EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY SSGGEDGYVR IHYFDPQYFE FEFEA

His-Tag

Baculovirus

Met1

Recombinant Protein

Q13347

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