Recombinant human Caspase-3 protein | NKMAX ATGP3874 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Caspase-3 protein

catalog :

ATGP3874

quantity :

10 ug

price :

235 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Caspase-3 protein

Domain :

29-277aa

Catalog number (SKU) :

ATGP3874

NCBI :

NP_116786

Description :

Caspase-3, also known as caspase-3 isoform a, is a member of the cysteine-aspartic acid protease (caspase) family of enzymes. This protein is most abundant in cell lines of lymphocytic origin. Also, it activates caspases 6 and 7 and the protein itself is processed and is activated by caspases 8, 9, and 10. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits that dimerize to form the active enzyme. Members of the caspase family of proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Recombinant Human Caspase-3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

CASP3, CPP32, CPP32B, SCA-1, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM HEPES buffer (pH 7.4) containing 0.1M NaCl, 1mM EDTA, 1mM DTT, 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Walsh JG., et al, (2008) Proc. Natl. Scad. Sci. USA 105:12815.;;Fernandes-Alnemri T., et al, (1994) J. Biol. Chem. 269:30761-30764.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 5,000pmol/min/ug. One unit will liberate 1pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.

Applications :

SDS-PAGE,Enzyme Activity

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

29.4 kDa (256aa)ns)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 5,000 pmol/min/ug. One unit will liberate 1 pmole of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C.

Amino Acid Sequence :

MSGISLDNSY KMDYPEMGLC IIINNKNFHK STGMTSRSGT DVDAANLRET FRNLKYEVRN KNDLTREEIV ELMRDVSKED HSKRSSFVCV LLSHGEEGII FGTNGPVDLK KITNFFRGDR CRSLTGKPKL FIIQACRGTE LDCGIETDSG VDDDMACHKI PVEADFLYAY STAPGYYSWR NSKDGSWFIQ SLCAMLKQYA DKLEFMHILT RVNRKVATEF ESFSFDATFH AKKQIPCIVS MLTKELYFYH

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ser29

Product Type Name :

Recombinant Protein

UniProt No. :

P42574

Internal Search Terms :

Caspase3,ATGP3874-10ug,ATGP3874-20ug,ATGP3874-50ug,ATGP3874-100ug,ATGP3874-250ug,ATGP3874-500ug,ATGP3874-1mg,ATGP3874-10,ATGP3874-20,ATGP3874-50,ATGP3874-100,ATGP3874-250,ATGP3874-500,ATGP3874-1

more info or order :

NKMAX product webpage