Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein | NKMAX ATGP3870 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein

catalog :

ATGP3870

quantity :

10 ug

price :

140 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein

Domain :

1-370aa

Catalog number (SKU) :

ATGP3870

NCBI :

NP_034533

Description :

Hydroxyacid Oxidase-1/HAO-1, also known as hydroxyacid oxidase 1, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on two carbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternative Names :

(S)-2-hydroxy-acid oxidase; EC 1.1.3.15, Glycolate oxidase, GOX, GOX1MGC142227; GOXMGC142225, HAO1, HAO-1, HAOX1, hydroxyacid oxidase (glycolate oxidase) 1, hydroxyacid oxidase 1, Hydroxyacid Oxidase1, Hydroxyacid Oxidase-1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pennati A., et al. (2009) J. Biol. Chem. 284(45):31214-22.;; Murray MS., et al. (2008) Biochemistry. 47(8):2439-49

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Signal Transduction

Molecular Weight :

43.4 kDa (393aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 1,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C

Amino Acid Sequence :

MLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9WU19

Internal Search Terms :

Hydroxyacid Oxidase1, HAO1,ATGP3870-10ug,ATGP3870-20ug,ATGP3870-50ug,ATGP3870-100ug,ATGP3870-250ug,ATGP3870-500ug,ATGP3870-1mg,ATGP3870-10,ATGP3870-20,ATGP3870-50,ATGP3870-100,ATGP3870-250,ATGP3870-500,ATGP3870-1

more info or order :

NKMAX product webpage