protein

Recombinant human IL-21 protein

ATGP3861

10 ug

180 USD

Recombinant human IL-21 protein

30-162 aa

ATGP3861

NP_068575

IL-21, also known as Interleukin-21, is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumor responses. It has broad immunoregulatory activity and can modulate both humoral and cell-mediated responses. It is important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. It is produced by activated T cell, and it influences proliferation of T and B cells and cytolytic activity of natural killer cells. It may be a critical factor in the control of persistent viral infections. It has a potential predicating significance for survival Time in patients in the development of sporadic CRC. It binds to the IL-21R and can activate janus kinase JAK1, JAK3, STAT1, and STAT3 in EDgamma-16 cells. Recombinant human IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.

Interleukin-21 isoform 1, CVID11, IL-21, Za11

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gui G., et al. (2017) Clin Immunol. 183:266-272.;;Parrish-Novak J., et al. (2000) Nature. 408(6808):57-63.

> 95% by SDS-PAGE

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤4ng/ml.

SDS-PAGE, Bioactivity

Human

Growth factors

15.5 kDa (134aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human IL-21 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤4 ng/ml.

MQGQDRHMIR MRQLIDIVDQ LKNYVNDLVP EFLPAPEDVE TNCEWSAFSC FQKAQLKSAN TGNNERIINV SIKKLKRKPP STNAGRRQKH RLTCPSCDSY EKKPPKEFLE RFKSLLQKMI HQHLSSRTHG SEDS

Non-Tagged

E.coli

Recombinant Protein

Q9HBE4

IL21,ATGP3861-10ug,ATGP3861-20ug,ATGP3861-50ug,ATGP3861-100ug,ATGP3861-250ug,ATGP3861-500ug,ATGP3861-1mg,ATGP3861-10,ATGP3861-20,ATGP3861-50,ATGP3861-100,ATGP3861-250,ATGP3861-500,ATGP3861-1

Park SJ, et al. A general toxicity and biodistribution study of human natural killer cells by single or repeated intravenous dose in severe combined immune deficient mice. (Toxicol Res. 2022){https://doi.org/10.1007/s43188-022-00138-0}