Recombinant bovine TNF-alpha protein | NKMAX ATGP3857 product information

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product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant bovine TNF-alpha protein

catalog :

ATGP3857

quantity :

20 ug

price :

170 USD

product information

Product Type :

Proteins

Item Name :

Recombinant bovine TNF-alpha protein

Domain :

78-234aa

Catalog number (SKU) :

ATGP3857

NCBI :

NP_776391

Description :

TNF-alpha, also known as tumor necrosis factor, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Bovine TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Tumor necrosis factor, TNF-a, TNF, TNFa

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bojarojc-Nosowicz B., et al (2015). Pol J Vet Sci. 18(3): 533-9.;;Lendez PA., et al (2015). Arch Virol. 160(8): 2001-7.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 15ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Bovine

Gene Category :

Hormones & Cytokines

Molecular Weight :

17.5 kDa (158aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Bovine TNF-alpha stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cells. The ED50 range ≤ 15ng/ml.

Bioassay Procedure :

MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q06599

Internal Search Terms :

TNF-a, TNF-alphaTumor necrosis factor, TNF-a, TNF, TNFa,ATGP3857-10ug,ATGP3857-20ug,ATGP3857-50ug,ATGP3857-100ug,ATGP3857-250ug,ATGP3857-500ug,ATGP3857-1mg,ATGP3857-10,ATGP3857-20,ATGP3857-50,ATGP3857-100,ATGP3857-250,ATGP3857-500,ATGP3857-1

Size 2 :

20 ug

List Price 2 (USD) :

170 USD