Recombinant human GalNAc Transferase 1/GALNT1 protein | NKMAX ATGP3850 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human GalNAc Transferase 1/GALNT1 protein

catalog :

ATGP3850

quantity :

20 ug

price :

200 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human GalNAc Transferase 1/GALNT1 protein

Domain :

41-559aa

Catalog number (SKU) :

ATGP3850

NCBI :

NP_065207

Description :

GALNT1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the GalNAc-T family of enzymes. It utilizes UDP-GalNAc as the nucleotide donor substrate to modify serine or threonine residues of proteins trafficking through the secretory pathway. Structurally, GalNAc-Ts consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant human GALNT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Polypeptide N-acetylgalactosaminyltransferase 1, Polypeptide GalNAc transferase 1, GalNAc-T1, pp-GaNTase 1, Protein-UDP acetylgalactosaminyltransferase 1, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Gerken, T.A. et al. (2011) J. Biol. Chem. 286:14493-14507.;;Partt, M.R. et al. (2004) Chem. Biol. 11:1009-1016.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that transfer 1.0pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute per minute at pH 8.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

60.4 kDa (528aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that transfer 1.0 pmole of GalNAc from UDP-GalNAc to peptide EA2 per minute per minute at pH 8.0 at 37C.

Amino Acid Sequence :

GLPAGDV LEPVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSKGQVITF LDAHCECTVG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRVGLRHKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCNGSRSQQ WLLRNVTLPE IF

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

Q10472

Internal Search Terms :

GALNT1, GALNT1Polypeptide N-acetylgalactosaminyltransferase 1, GALNT1, GALNAC-T1,ATGP3850-10ug,ATGP3850-20ug,ATGP3850-50ug,ATGP3850-100ug,ATGP3850-250ug,ATGP3850-500ug,ATGP3850-1mg,ATGP3850-10,ATGP3850-20,ATGP3850-50,ATGP3850-100,ATGP3850-250,ATGP3850-500,ATGP3850-1

more info or order :

NKMAX product webpage