Recombinant human Hydroxyacid Oxidase-1/HAO-1 protein | NKMAX ATGP3841 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Hydroxyacid Oxidase-1/HAO-1 protein

catalog :

ATGP3841

quantity :

10 ug

price :

150 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Hydroxyacid Oxidase-1/HAO-1 protein

Domain :

1-370aa

Catalog number (SKU) :

ATGP3841

NCBI :

NP_060015

Description :

HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant human HAO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Hydroxyacid oxidase 1, GOX (glycolate oxidase), GOX1, HAOX1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.5M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pennati A., et al. (2009) J. Biol. Chem. 284:31214.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 3,000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.

Applications :

SDS-PAGE,Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

45.0 kDa (406aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 3,000 pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C.

Amino Acid Sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMLPR LICINDYEQH AKSVLPKSIY DYYRSGANDE ETLADNIAAF SRWKLYPRML RNVAETDLST SVLGQRVSMP ICVGATAMQR MAHVDGELAT VRACQSLGTG MMLSSWATSS IEEVAEAGPE ALRWLQLYIY KDREVTKKLV RQAEKMGYKA IFVTVDTPYL GNRLDDVRNR FKLPPQLRMK NFETSTLSFS PEENFGDDSG LAAYVAKAID PSISWEDIKW LRRLTSLPIV AKGILRGDDA REAVKHGLNG ILVSNHGARQ LDGVPATIDV LPEIVEAVEG KVEVFLDGGV RKGTDVLKAL ALGAKAVFVG RPIVWGLAFQ GEKGVQDVLE ILKEEFRLAM ALSGCQNVKV IDKTLVRKNP LAVSKI

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9UJM8

Internal Search Terms :

Hydroxyacid Oxidase1, HAO1, Hydroxyacid oxidase 1, GOX (glycolate oxidase), GOX1, HAOX1,ATGP3841-10ug,ATGP3841-20ug,ATGP3841-50ug,ATGP3841-100ug,ATGP3841-250ug,ATGP3841-500ug,ATGP3841-1mg,ATGP3841-10,ATGP3841-20,ATGP3841-50,ATGP3841-100,ATGP3841-250,ATGP3841-500,ATGP3841-1

more info or order :

NKMAX product webpage