Recombinant mouse Aldehyde dehydrogenase 2/ALDH2 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Aldehyde dehydrogenase 2/ALDH2 protein

catalog :

ATGP3839

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Aldehyde dehydrogenase 2/ALDH2 protein

Domain :

20-519aa

Catalog number (SKU) :

ATGP3839

NCBI :

NP_033786

Description :

Aldh2 also known as Aldehyde dehydrogenase, mitochondrial. Aldh2 belongs to the aldehyde dehydrogenase family which catalyzes the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant mouse ALDH2 was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase 2 family member, AHD-M1, ALDH class 2, ALDH-E2, ALDHI, Ahd-1, ALDM

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chang C., et al. (1994) Gene. 148:331-336.;;Chen M., et al. (1994) Mol. Pharmacol. 46:88-96.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 180pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25℃.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Metabolism

Molecular Weight :

56.8 kDa (523aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 180pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25℃.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSSAAATSA VPAPNHQPEV FCNQIFINNE WHDAVSRKTF PTVNPSTGEV ICQVAEGNKE DVDKAVKAAR AAFQLGSPWR RMDASDRGRL LYRLADLIER DRTYLAALET LDNGKPYVIS YLVDLDMVLK CLRYYAGWAD KYHGKTIPID GDFFSYTRHE PVGVCGQIIP WNFPLLMQAW KLGPALATGN VVVMKVAEQT PLTALYVANL IKEAGFPPGV VNIVPGFGPT AGAAIASHEG VDKVAFTGST EVGHLIQVAA GSSNLKRVTL ELGGKSPNII MSDADMDWAV EQAHFALFFN QGQCCCAGSR TFVQENVYDE FVERSVARAK SRVVGNPFDS RTEQGPQVDE TQFKKILGYI KSGQQEGAKL LCGGGAAADR GYFIQPTVFG DVKDGMTIAK EEIFGPVMQI LKFKTIEEVV GRANDSKYGL AAAVFTKDLD KANYLSQALQ AGTVWINCYD VFGAQSPFGG YKMSGSGREL GEYGLQAYTE VKTVTVKVPQ KNS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P47738

Internal Search Terms :

Aldh2, Aldehyde dehydrogenase mitochondrial, Aldehyde dehydrogenase, mitochondrial, Ahd-5, Ahd5, Ahd 5, Aldehyde dehydrogenase 2 family member, AHD-M1, ALDH class 2, ALDH-E2, ALDHI, Ahd-1, ALDM, AHD M1, ALDH E2, AHDM1, ALDHE2, ATGP3839-10ug, ATGP3839-20ug, ATGP3839-50ug, ATGP3839-100ug, ATGP3839-250ug, ATGP3839-500ug, ATGP3839-1mg, ATGP3839-010, ATGP3839-020, ATGP3839-050, ATGP3839-100, ATGP3839-250, ATGP3839-500, ATGP3839-01M

more info or order :

NKMAX product webpage