Recombinant human Arylsulfatase A/ARSA protein | NKMAX ATGP3826 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Arylsulfatase A/ARSA protein

catalog :

ATGP3826

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Arylsulfatase A/ARSA protein

Domain :

21-509aa

Catalog number (SKU) :

ATGP3826

NCBI :

NP_000478

Description :

ARSA, also known as arylsulfatase A isoform a, is a member of the sulfatase family. It hydrolyzes cerebroside sulfate, namely cerebroside 3-sulfate. It is found in many tissues, but predominantly in myelin and kidney. This protein is activated by a posttranslational modification (PTM) with the oxidation of cysteine to formylglycine. It could be helpful in diagnosis of lung and central nervous system cancer through the serum level of this protein. The ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms. Recombinant human ARSA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

ASA, Cerebroside-sulfatase, metachromatic leucodystrophy(MLD)

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shahzad MA., et al, (2017) J Mol Neurosci. 63:84-90.;;Cesani M., et al, (2017) Hum Mutat. 37:16-27.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2,500pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

53 kDa (498aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 2,500 pmol/min/ug, and defined as the amount of enzyme that hydrolyze 4-Nitrocatechol at pH 5.0 at 37C.

Amino Acid Sequence :

RPPNIVL IFADDLGYGD LGCYGHPSST TPNLDQLAAG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRM GMYPGVLVPS SRGGLPLEEV TVAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPA TPCDGGCDQG LVPIPLLANL SVEAQPPWLP GLEARYMAFA HDLMADAQRQ DRPFFLYYAS HHTHYPQFSG QSFAERSGRG PFGDSLMELD AAVGTLMTAI GDLGLLEETL VIFTADNGPE TMRMSRGGCS GLLRCGKGTT YEGGVREPAL AFWPGHIAPG VTHELASSLD LLPTLAALAG APLPNVTLDG FDLSPLLLGT GKSPRQSLFF YPSYPDEVRG VFAVRTGKYK AHFFTQGSAH SDTTADPACH ASSSLTAHEP PLLYDLSKDP GENYNLLGGV AGATPEVLQA LKQLQLLKAQ LDAAVTFGPS QVARGEDPAL QICCHPGCTP RPACCHCPDP HA

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P15289

Internal Search Terms :

ARSA, Arylsulfatase A isoform a, ARSA, ASA, MLD,ATGP3826-10ug,ATGP3826-20ug,ATGP3826-50ug,ATGP3826-100ug,ATGP3826-250ug,ATGP3826-500ug,ATGP3826-1mg,ATGP3826-10,ATGP3826-20,ATGP3826-50,ATGP3826-100,ATGP3826-250,ATGP3826-500,ATGP3826-1

more info or order :

NKMAX product webpage