product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human Proinsulin protein
catalog :
ATGP3817
quantity :
10 ug
price :
122 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human Proinsulin protein
Domain :
25-110aa
Catalog number (SKU) :
ATGP3817
NCBI :
NP_000198
Description :
INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names :
Insulin preproprotein, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
Concentration :
0.5mg/ml (determined by absorbance at 280nm)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Sun J., et al. (2015) Mol Aspects Med. 42:105-118.;;Liu M., et al. (2014) Vitam Horm. 95:35-62.
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4ug/ml.
Applications :
SDS-PAGE, Bioactivity
Antigen Species :
Human
Gene Category :
Signal Transduction
Molecular Weight :
11.8 kDa (109aa) confirmed by MALDI-TOF
Endotoxin :
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment and Pics :
Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4ug/ml.
Amino Acid Sequence :
FVNQHLC GSHLVEALYL VCGERGFFYT PKTRREAEDL QVGQVELGGG PGAGSLQPLA LEGSLQKRGI VEQCCTSICS LYQLENYCN
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P01308
Internal Search Terms :
Proinsulin, Insulin preproprotein, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10,ATGP3817-10ug,ATGP3817-20ug,ATGP3817-50ug,ATGP3817-100ug,ATGP3817-250ug,ATGP3817-500ug,ATGP3817-1mg,ATGP3817-10,ATGP3817-20,ATGP3817-50,ATGP3817-100,ATGP3817-250,ATGP3817-500,ATGP3817-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.