Recombinant human Ephrin-B1 protein | NKMAX ATGP3800 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Ephrin-B1 protein

catalog :

ATGP3800

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Ephrin-B1 protein

Domain :

28-237aa

Catalog number (SKU) :

ATGP3800

NCBI :

NP_004420

Description :

EFNB1, also known as ephrin-B1, is a member of Eph family. It binds to the receptor tyrosine kinases EPHB1 and EPHA1. This protein also binds Eph receptors residing on adjacent cells which affect signaling to neighboring cells. It plays a role in cellular migration, axon guidance, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis. Recombinant human EFNB1 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Ephrin B1, EFNB1, EFL-3, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EPLG2, craniofrontonasal syndrome, CFNS

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hu Y., et al, (2015) Arthritis Rheumatol. 67:1778-1788.;;Gibson J., et al, (2017) Dev Biol. 431:179-193.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human EphB2 (CAT# ATGP3493). The ED50 range ≤ 100 ng/ml. Measured by its binding ability in a functional ELISA with Human EPHB1 (CAT# ATGP4038). The ED50 range ≤ 40 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Cardiovascular

Molecular Weight :

50.3 kDa (452aa)

Endotoxin :

< 0.1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human EphB2 (CAT# ATGP3493) is coated at 2 ug/ml (100 ul/well) can bind Human Ephrin-B1. The ED50 range ≤ 100 ng/ml.Human EPHB1 (CAT# ATGP4038) is coated at 2 ug/ml (100 ul/well) can bind Human Ephrin-B1. The ED50 range ≤ 40 ng/ml.{2004014847361565.jpg}

Amino Acid Sequence :

LAKNLEP VSWSSLNPKF LSGKGLVIYP KIGDKLDIIC PRAEAGRPYE YYKLYLVRPE QAAACSTVLD PNVLVTCNRP EQEIRFTIKF QEFSPNYMGL EFKKHHDYYI TSTSNGSLEG LENREGGVCR TRTMKIIMKV GQDPNAVTPE QLTTSRPSKE ADNTVKMATQ APGSRGSLGD SDGKHETVNQ EEKSGPGASG GSSGDPDGFF NSK

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P98172

Internal Search Terms :

Ephrin-B1, Ephrin-B1, EFNB1, CFND, CFNS, EFB1, EFL3, Elk-L, EPLG2, LERK2,ATGP3800-10ug,ATGP3800-20ug,ATGP3800-50ug,ATGP3800-100ug,ATGP3800-250ug,ATGP3800-500ug,ATGP3800-1mg,ATGP3800-10,ATGP3800-20,ATGP3800-50,ATGP3800-100,ATGP3800-250,ATGP3800-500,ATGP3800-1

more info or order :

NKMAX product webpage