protein

Recombinant human IL-34 protein

ATGP3796

10 ug

160 USD

Recombinant human IL-34 protein

21-242aa

ATGP3796

NP_689669.1

IL34, also known as interleukin-34 isoform 1, is a secreted cytokine. This protein is expressed in a variety of tissues richest in the spleen and stimulates the proliferation of monocytes. In a functional study, IL-34, such as CSF-1, another ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1 / 2) in human monocytes. In addition, it promoted the formation of macrophage precursor colonization units-macrophages (CFU-M) in human bone marrow cultures. Recombinant human IL34, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Interleukin-34 isoform 1, IL34, C16orf77, IL-34

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.15M NaCl, 0.1mM PMSF, 1mM EDTA, 30% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Franze E, et al. (2015) Clin Sci (Lond). 129:271-280.;;Lin, H., et al. (2008) Science 320:807-811.

> 90% by SDS-PAGE

SDS-PAGE

Human

Immunology

26.3 kDa (231aa)

< 1 EU per 1ug of protein (determined by LAL method)

NEPLEMW PLTQNEECTV TGFLRDKLQY RSRLQYMKHY FPINYKISVP YEGVFRIANV TRLQRAQVSE RELRYLWVLV SLSATESVQD VLLEGHPSWK YLQEVQTLLL NVQQGLTDVE VSPKVESVLS LLNAPGPNLK LVRPKALLDN CFRVMELLYC SCCKQSSVLN WQDCEVPSPQ SCSPEPSLQY AATQLYPPPP WSPSSPPHST GSVRPVRAQG EGLLP

His-Tag

Baculovirus

Ala

Recombinant Protein

Q6ZMJ4

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