Recombinant human M-CSF R/CD115 protein | NKMAX ATGP3794 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human M-CSF R/CD115 protein

catalog :

ATGP3794

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human M-CSF R/CD115 protein

Domain :

20-517aa

Catalog number (SKU) :

ATGP3794

NCBI :

NP_005202.2

Description :

CSF1R, also known as macrophage colony-stimulating factor 1 receptor, is a member of the type 3 subfamily of receptor tyrosine kinases. It is expressed primarily on cells of the monocyte and macrophage lineage, stem cells, and in the developing placenta and mediates most of the biological effects of this cytokine. This protein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. CSF1 and this receptor were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role of CSF1/CSF1R in normal mammary gland development is very intriguing because this relationship has also been found in the biology of breast cancer in which abnormal expression of CSF1 and its receptor. Also, increased levels of CSF1R are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Recombinant human CSF1R, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Macrophage colony-stimulating factor 1 receptor, CSF1R, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, HDLS, M-CSF-R

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yeung YG., et al, (1998) J Biol Chem. 273:17128-17137.;;Sapi E., et al, (1999) Proc Soc Exp Biol Med. 220:1-8.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured by its ability to inhibit proliferation using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤100ng/ml with Human M-CSF.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Angiogenesis

Molecular Weight :

82.1 kDa (737aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Measured by its ability to inhibit proliferation using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤100 ng/ml with Human M-CSF.

Amino Acid Sequence :

IPVIEPSVPE LVVKPGATVT LRCVGNGSVE WDGPPSPHWT LYSDGSSSIL STNNATFQNT GTYRCTEPGD PLGGSAAIHL YVKDPARPWN VLAQEVVVFE DQDALLPCLL TDPVLEAGVS LVRVRGRPLM RHTNYSFSPW HGFTIHRAKF IQSQDYQCSA LMGGRKVMSI SIRLKVQKVI PGPPALTLVP AELVRIRGEA AQIVCSASSV DVNFDVFLQH NNTKLAIPQQ SDFHNNRYQK VLTLNLDQVD FQHAGNYSCV ASNVQGKHST SMFFRVVESA YLNLSSEQNL IQEVTVGEGL NLKVMVEAYP GLQGFNWTYL GPFSDHQPEP KLANATTKDT YRHTFTLSLP RLKPSEAGRY SFLARNPGGW RALTFELTLR YPPEVSVIWT FINGSGTLLC AASGYPQPNV TWLQCSGHTD RCDEAQVLQV WDDPYPEVLS QEPFHKVTVQ SLLTVETLEH NQTYECRAHN SVGSGSWAFI PISAGAHTHP PDEFLFTP

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ile20

Product Type Name :

Recombinant Protein

UniProt No. :

P07333

Internal Search Terms :

M-CSF CD115,ATGP3794-10ug,ATGP3794-20ug,ATGP3794-50ug,ATGP3794-100ug,ATGP3794-250ug,ATGP3794-500ug,ATGP3794-1mg,ATGP3794-10,ATGP3794-20,ATGP3794-50,ATGP3794-100,ATGP3794-250,ATGP3794-500,ATGP3794-1

more info or order :

NKMAX product webpage