Recombinant S. Japonicum Glutathione S-transferase/GST protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant S. Japonicum Glutathione S-transferase/GST protein

catalog :

ATGP3792

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant S. Japonicum Glutathione S-transferase/GST protein

Domain :

1-218aa

Catalog number (SKU) :

ATGP3792

NCBI :

AAB59203

Description :

GST, also known as, Glutathione S-transferase, represents a major group of detoxification enzymes. GST acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. GST was originally separated from Schistosomajaponicum but currently isolated from recombinant E. coli source. Recombinant Schistosoma japonicum GST was expressed in E. coli and purified by conventional chromatography techniques.

Alternative Names :

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hayes JD., et al. (1995) Crit Rev BiochemMol Biol. 30(6):445-600.;;Smith DB., et al. (1988) Mol Biochem Parasitol. 27(2-3):249-56.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 30unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Schistosoma japonicum

Gene Category :

Fusion Tag & Detection

Molecular Weight :

25.4 kDa (218aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 30 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Amino Acid Sequence :

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P08515

Internal Search Terms :

Glutathione S-Transferase, GST, Glutathione S-Transferase, Sj26 antigen,ATGP3792-10ug,ATGP3792-20ug,ATGP3792-50ug,ATGP3792-100ug,ATGP3792-250ug,ATGP3792-500ug,ATGP3792-1mg,ATGP3792-10,ATGP3792-20,ATGP3792-50,ATGP3792-100,ATGP3792-250,ATGP3792-500,ATGP3792-1

more info or order :

NKMAX product webpage