protein

Recombinant human Serpin C1/Antithrombin-III protein

ATGP3791

5 ug

105 USD

Recombinant human Serpin C1/Antithrombin-III protein

1-546aa

ATGP3791

NP_000479

SERPINC1, also known as antithrombin-III, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. It is one of the most important coagulation inhibitors and is the basic enzyme for the therapeutic action of heparin. In common with SerpinA5 and D1, the inhibitory activity of SerpinC1 dramatically increases the presence of heparin and other glycosaminoglycans. It mediates the promotion of prostaglandin release, inhibitors of leukocyte activation and down-regulation of many inflammatory cytokines. This protein has anti-inflammatory properties besides anticoagulant. Recombinant human SERPINC1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Antithrombin-III, SERPINC1, AT3, AT3D, ATIII, THPH7

0.5mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Caglikulekci M., et al. (2004) Ann Chir. 129:273-277.;;Chuang, Y.-J., et al. (2001) Biochemistry 40:6670-6679.

> 90% by SDS-PAGE

Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1nM.

Enzyme Activity,SDS-PAGE

Human

Cardiovascular

50.1 kDa (441aa)

< 1 EU per 1ug of protein (determined by LAL method)

Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 1nM.

ADPHGSPVDI CTAKPRDIPM NPMCIYRSPE KKATEDEGSE QKIPEATNRR VWELSKANSR FATTFYQHLA DSKNDNDNIF LSPLSISTAF AMTKLGACND TLQQLMEVFK FDTISEKTSD QIHFFFAKLN CRLYRKANKS SKLVSANRLF GDKSLTFNET YQDISELVYG AKLQPLDFKE NAEQSRAAIN KWVSNKTEGR ITDVIPSEAI NELTVLVLVN TIYFKGLWKS KFSPENTRKE LFYKADGESC SASMMYQEGK FRYRRVAEGT QVLELPFKGD DITMVLILPK PEKSLAKVEK ELTPEVLQEW LDELEEMMLV VHMPRFRIED GFSLKEQLQD MGLVDLFSPE KSKLPGIVAE GRDDLYVSDA FHKAFLEVNE EGSEAAASTA VVIAGRSLNP NRVTFKANRP FLVFIREVPL NTIIFMGRVA NPCVKHHHHH H

His-Tag

Baculovirus

Ala

Recombinant Protein

P01008

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