Recombinant human Granzyme B protein | NKMAX ATGP3774 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Granzyme B protein

catalog :

ATGP3774

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Granzyme B protein

Domain :

19-247aa

Catalog number (SKU) :

ATGP3774

NCBI :

NP_004122.2

Description :

GZMB, also known as granzyme B isoform 1, is member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Tis protein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. Also, it processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. Recombinant human GZMB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Granzyme B isoform 1, GZMB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Poe M., et al, (1991) J. Biol. Chem. 266:98-103.;;Xu W., et al, (2014) Eur. J. Immunol. 44(1) 275-284.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Apoptosis

Molecular Weight :

26.5 kDa (235aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Amino Acid Sequence :

GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRY

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Gly19

Product Type Name :

Recombinant Protein

UniProt No. :

P10144

Internal Search Terms :

Granzyme B, Granzyme B isoform 1, GZMB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT,ATGP3774-10ug,ATGP3774-20ug,ATGP3774-50ug,ATGP3774-100ug,ATGP3774-250ug,ATGP3774-500ug,ATGP3774-1mg,ATGP3774-10,ATGP3774-20,ATGP3774-50,ATGP3774-100,ATGP3774-250,ATGP3774-500,ATGP3774-1

more info or order :

NKMAX product webpage