Recombinant mouse IL-12 p70 protein | NKMAX ATGP3739 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse IL-12 p70 protein

catalog :

ATGP3739

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse IL-12 p70 protein

Domain :

23-335aa(p40)/23-215aa(p35)

Catalog number (SKU) :

ATGP3739

NCBI :

NP_001290173(p40)/NP_032377(p35)

Description :

IL12b/IL12a, also known as interleukin 12 (subunit beta/alpha), is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. It is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. As it is associated with IL23A to form the IL-23 interleukin, it induces autoimmune inflammation and is responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Recombinant mouse IL12b/IL12a protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhang X., et al, (2015) J Immunol. 195:1665-1675.;; Zamani A., et al, (2017) Immune Netw. 17:186-191.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Mouse

Gene Category :

Hormones & Cytokines

Molecular Weight :

35.7 kDa (p40, 313aa);; 22.5 kDa (p35, 199aa);;The heterodimeric Il12 has a predicted Molecular weight of 58.3 kDa (512aa).;;Molecular weight on SDS-PAGE will appear higher

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Mouse IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/ml.

Amino Acid Sequence :

IL12B(p40);; MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS;; IL12A(p35);; RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Met23(p40)/Arg23(p35)

Product Type Name :

Recombinant Protein

UniProt No. :

P43432(p40)/P43431(p35)

Internal Search Terms :

IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin-12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2, IL12, IL-12, Interleukin12, Interleukin 12, IL 12, Interleukin 12 alpha chain, Interleukin 12 beta chain, ATGP3739-10ug, ATGP3739-20ug, ATGP3739-50ug, ATGP3739-100ug, ATGP3739-250ug, ATGP3739-500ug, ATGP3739-1mg, ATGP3739-010, ATGP3739-020, ATGP3739-050, ATGP3739-100, ATGP3739-250, ATGP3739-500, ATGP3739-01M

more info or order :

NKMAX product webpage