Recombinant rat TNF-alpha protein | NKMAX ATGP3736 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant rat TNF-alpha protein

catalog :

ATGP3736

quantity :

10 ug

price :

45 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant rat TNF-alpha protein

Domain :

80-235aa

Catalog number (SKU) :

ATGP3736

NCBI :

NP_036807

Description :

Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. Recombinant rat Tnf, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Furuya T., et al. (2001) Genes Immun. 2:229-232.;; Warle M.C., et al. (2005) Transpl. Immunol. 14:77-82.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Rat

Gene Category :

Hormones & Cytokines

Molecular Weight :

19.9 kDa (181aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Rat TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Amino Acid Sequence :

LRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P16599

Internal Search Terms :

TNF-a,ATGP3736-10ug,ATGP3736-20ug,ATGP3736-50ug,ATGP3736-100ug,ATGP3736-250ug,ATGP3736-500ug,ATGP3736-1mg,ATGP3736-10,ATGP3736-20,ATGP3736-50,ATGP3736-100,ATGP3736-250,ATGP3736-500,ATGP3736-1

more info or order :

NKMAX product webpage