protein

Recombinant human Histamine N-Methyltransferase/HNMT protein

ATGP3727

5 ug

105 USD

Recombinant human Histamine N-Methyltransferase/HNMT protein

1-292aa

ATGP3727

AAH20677

HNMT also known as Histamine N-methyltransferase. HNMT is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT inactivates histamine by N-methylation. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme histamine N-methyltransferase (HNMT) in bronchus, kidney, and the central nervous system. It plays an important role in degrading histamine and in regulating the airway response to histamine. Recombinant human HNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Histamine N-methyltransferase isoform 1, HMT, HNMT-S1, HNMT-S2, MRT51

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Garcia-Martin E., et al. (2009) Pharmacogenomics. 10(5):867-83.;; Palikhe NS., et al. (2008) J Clin Pharm Ther. 33(5):465-72.

> 90% by SDS-PAGE

Specific activity is > 200nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0nmole of methyl group per minute at 37C

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

37.4 kDa (328aa) confirmed by MALDI-TOF

Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C

MASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA

His-Tag

E.coli

Recombinant Protein

P50135

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