Recombinant mouse UDP-glucose dehydrogenase/UGDH protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse UDP-glucose dehydrogenase/UGDH protein

catalog :

ATGP3725

quantity :

5 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse UDP-glucose dehydrogenase/UGDH protein

Domain :

1-493aa

Catalog number (SKU) :

ATGP3725

NCBI :

NP_033492

Description :

Ugdh, also known as UDP-glucose 6-dehydrohenase, is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant mouse Ugdh, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM MES buffer (pH 5.0) containing 20% glycerol, 150mM NaCl, 1mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bontemps Y., et al. (2003) J Biol Chem. 278(24):21566-75.;; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70(2):401-10.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Cell Cycle

Molecular Weight :

57.2 kDa (516aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMVEIKKI CCIGAGYVGG PTCSVIAHMC PEIRVTVVDV NEARINAWNS PTLPIYEPGL KEVVESCRGK NLFFSTNIDD AIREADLVFI SVNTPTKTYG MGKGRAADLK YIEACARRIV QNSNGYKIVT EKSTVPVRAA ESIRRIFDAN TKPNLNLQVL SNPEFLAEGT AIKDLKNPDR VLIGGDETPE GQKAVRALCA VYEHWVPKEK ILTTNTWSSE LSKLAANAFL AQRISSINSI SALCEATGAD VEEVATAIGM DQRIGNKFLK ASVGFGGSCF QKDVLNLVYL CEALNLPEVA RYWQQVIDMN DYQRRRFASR IIDSLFNTVT DKKIAILGFA FKKDTGDTRE SSSIYISKYL MDEGAHLHIY DPKVPREQIV VDLSHPGVSA DDQVSRLVTI SKDPYEACDG AHALVICTEW DMFKELDYER IHKKMLKPAF IFDGRRVLDG LHSELQTIGF QIETIGKKVS SKRIPYTPGE IPKFSLQDPP NKKPKV

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O70475

Internal Search Terms :

Ugdh, UDP-glucose 6-dehydrohenase, Udpgdh,ATGP3725-10ug,ATGP3725-20ug,ATGP3725-50ug,ATGP3725-100ug,ATGP3725-250ug,ATGP3725-500ug,ATGP3725-1mg,ATGP3725-10,ATGP3725-20,ATGP3725-50,ATGP3725-100,ATGP3725-250,ATGP3725-500,ATGP3725-1

more info or order :

NKMAX product webpage