Recombinant human IFN-gamma/IFNG protein | NKMAX ATGP3723 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IFN-gamma/IFNG protein

catalog :

ATGP3723

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IFN-gamma/IFNG protein

Domain :

24-161aa

Catalog number (SKU) :

ATGP3723

NCBI :

NP_000610

Description :

IFNG, also known as Interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Nature human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Interferon gamma, Immune interferon, IFG, IFI, IMD69

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Arakawa T., et al. (1987) Biochemistry. 26(17):5428-32;; Rinderknecht E., et al. (1984) J Biol Chem. 259(11):6790-7

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤5ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

18.4 kDa (159aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IFN-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤5 ng/ml.

Amino Acid Sequence :

QDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P01579

Internal Search Terms :

IFN-g,ATGP3723-10ug,ATGP3723-20ug,ATGP3723-50ug,ATGP3723-100ug,ATGP3723-250ug,ATGP3723-500ug,ATGP3723-1mg,ATGP3723-10,ATGP3723-20,ATGP3723-50,ATGP3723-100,ATGP3723-250,ATGP3723-500,ATGP3723-1

more info or order :

NKMAX product webpage