Recombinant mouse Malate dehydrogenase 2/MDH2 protein | NKMAX ATGP3722 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Malate dehydrogenase 2/MDH2 protein

catalog :

ATGP3722

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Malate dehydrogenase 2/MDH2 protein

Domain :

25-338aa

Catalog number (SKU) :

ATGP3722

NCBI :

NP_032643

Description :

Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Malate dehydrogenase, mitochondrial, MDH, Mdh-2, Mor-1, Mor1

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wang L., et al. (2010) Dev Dyn. 239(3):954-64;; Chang AK., et al. (2002) J Basic Microbiol. 42(4):238-45.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 800unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Signal Transduction

Molecular Weight :

35.4 kDa (335aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P08249

Internal Search Terms :

Mdh2, Malate dehydrogenase, mitochondrial, MDH, Mdh-2, Mor-1, Mor1,ATGP3722-10ug,ATGP3722-20ug,ATGP3722-50ug,ATGP3722-100ug,ATGP3722-250ug,ATGP3722-500ug,ATGP3722-1mg,ATGP3722-10,ATGP3722-20,ATGP3722-50,ATGP3722-100,ATGP3722-250,ATGP3722-500,ATGP3722-1

more info or order :

NKMAX product webpage