Recombinant mouse AK1 protein | NKMAX ATGP3700 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse AK1 protein

catalog :

ATGP3700

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse AK1 protein

Domain :

1-210aa

Catalog number (SKU) :

ATGP3700

NCBI :

NP_067490.1

Description :

Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Adenylate kinase isoenzyme 1, Adenylate kinase 1, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase 1, Myokinase,

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Dzeja PP., et al. (2007) J Biol Chem. 282(43):31366-72.;; Ravera S., et al., (2015) Curr Eye Res. 32(3):249-57.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 150unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Metabolism

Molecular Weight :

25.5 kDa (233aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9R0Y5

Internal Search Terms :

Ak1, Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik,ATGP3700-10ug,ATGP3700-20ug,ATGP3700-50ug,ATGP3700-100ug,ATGP3700-250ug,ATGP3700-500ug,ATGP3700-1mg,ATGP3700-10,ATGP3700-20,ATGP3700-50,ATGP3700-100,ATGP3700-250,ATGP3700-500,ATGP3700-1

more info or order :

NKMAX product webpage