protein

Recombinant mouse Adenosine Kinase/ADK protein

ATGP3692

10 ug

105 USD

Recombinant mouse Adenosine Kinase/ADK protein

1-361aa

ATGP3692

NP_598840

Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

ADK, AK, adenosine kinase, Adenosine 5'-phosphotransferase

1mg/ml (determined by absorbance at 280nm)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 50mM NaCl, 1mM EDTA

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Chakraborty A., et al. (2002) J Biol Chem. 277(49: 47451-60.;; Sakowicz M., et al. (2001) Acta Biochim Pol. 48(3):745-54.

> 95% by SDS-PAGE

Specific activity is > 100pmol/min/ and is defined as the amount of enzyme that convert 1.0pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

SDS-PAGE, Enzyme Activity

Mouse

Enzymes & Proteases

42.5 kDa (384aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH

His-Tag

E.coli

Recombinant Protein

P55264

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