Recombinant mouse Enolase 1/ENO1 protein | NKMAX ATGP3691 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Enolase 1/ENO1 protein

catalog :

ATGP3691

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Enolase 1/ENO1 protein

Domain :

1-434aa

Catalog number (SKU) :

ATGP3691

NCBI :

NP_075608

Description :

Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, c-Myc promoter binding protein

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.1M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Plow EF., et al. (2009) Blood. 113(22):5371-2. ;; Bartholomeusz C., et al. (2008) Cancer Res. 68(22):9302-10.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 6,000pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Metabolism

Molecular Weight :

49.5 kDa (457aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

Amino Acid Sequence :

MSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P17182

Internal Search Terms :

Enolase1, Non-neural enolase, Alpha-Enolase, 0610008I15, AL022784, Eno-1, MBP-1,ATGP3691-10ug,ATGP3691-20ug,ATGP3691-50ug,ATGP3691-100ug,ATGP3691-250ug,ATGP3691-500ug,ATGP3691-1mg,ATGP3691-10,ATGP3691-20,ATGP3691-50,ATGP3691-100,ATGP3691-250,ATGP3691-500,ATGP3691-1

more info or order :

NKMAX product webpage