Recombinant human Nicotinamide N-Methyltransferase/NNMT protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Nicotinamide N-Methyltransferase/NNMT protein

catalog :

ATGP3681

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Nicotinamide N-Methyltransferase/NNMT protein

Domain :

1-264aa

Catalog number (SKU) :

ATGP3681

NCBI :

NP_006160

Description :

NNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Nicotinamide N-methyltransferase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Parsons RB., et al. (2002). J Neuropathol Exp Neurol. 61(2):111-24.;; Smith ML., et al. (1998). Biochim Biophys Acta. 1442(2-3):238-44.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 100nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0nmole of methyl group per minute at 37C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

37.7 kDa (284aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P40261

Internal Search Terms :

Nicotinamide N-Methyltransferase, NNMT, Nicotinamide N-methyltransferase,ATGP3681-10ug,ATGP3681-20ug,ATGP3681-50ug,ATGP3681-100ug,ATGP3681-250ug,ATGP3681-500ug,ATGP3681-1mg,ATGP3681-10,ATGP3681-20,ATGP3681-50,ATGP3681-100,ATGP3681-250,ATGP3681-500,ATGP3681-1

more info or order :

NKMAX product webpage