Recombinant human Glycine N-methyltransferase/GNMT protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Glycine N-methyltransferase/GNMT protein

catalog :

ATGP3679

quantity :

10 ug

price :

140 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Glycine N-methyltransferase/GNMT protein

Domain :

1-295aa

Catalog number (SKU) :

ATGP3679

NCBI :

NP_061833

Description :

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glycine N-methyltransferase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Liao YJ., et al. (2009) Int J Cancer.124(4):816-26.;; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23(9):1384-9.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 100nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0nmole of methyl group per minute at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

34.9 kDa (315aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Amino Acid Sequence :

MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q14749

Internal Search Terms :

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase,ATGP3679-10ug,ATGP3679-20ug,ATGP3679-50ug,ATGP3679-100ug,ATGP3679-250ug,ATGP3679-500ug,ATGP3679-1mg,ATGP3679-10,ATGP3679-20,ATGP3679-50,ATGP3679-100,ATGP3679-250,ATGP3679-500,ATGP3679-1

more info or order :

NKMAX product webpage