protein

Recombinant human HMGCL protein

ATGP3658

10 ug

80 USD

Recombinant human HMGCL protein

28-325aa

ATGP3658

NP_000182

HMGCL, also known as hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Multiple isoforms of the proteins are known due to alternative splicing. The major isoform (isoform 1) is most highly expressed in the liver whereas isoform 2 is found in energy-demanding tissues including the brain, heart, and skeletal muscle. Recombinant human HMGCL protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, HMGCL, HL

1mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Fu Z., et al, (2010) J Biol Chem. 285:26341-26349.;; Montgomery C., et al, (2011) Arch Biochem Biophys. 511:48-55.

> 90% by SDS-PAGE

SDS-PAGE

Human

Signal Transduction

32.5 kDa (305aa)

< 1 EU per 1ug of protein (determined by LAL method)

MTLPKRVKIV EVGPRDGLQN EKNIVSTPVK IKLIDMLSEA GLSVIETTSF VSPKWVPQMG DHTEVLKGIQ KFPGINYPVL TPNLKGFEAA VAAGAKEVVI FGAASELFTK KNINCSIEES FQRFDAILKA AQSANISVRG YVSCALGCPY EGKISPAKVA EVTKKFYSMG CYEISLGDTI GVGTPGIMKD MLSAVMQEVP LAALAVHCHD TYGQALANTL MALQMGVSVV DSSVAGLGGC PYAQGASGNL ATEDLVYMLE GLGIHTGVNL QKLLEAGNFI CQALNRKTSS KVAQATCKL

His-Tag

Baculovirus

Met

Recombinant Protein

P35914

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