Recombinant mouse TNF-alpha protein | NKMAX ATGP3651 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse TNF-alpha protein

catalog :

ATGP3651

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse TNF-alpha protein

Domain :

80-235aa

Catalog number (SKU) :

ATGP3651

NCBI :

NP_038721

Description :

TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705;; Pennica D., et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82:6060-6064

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.06ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Mouse

Gene Category :

Hormones & Cytokines

Additional Comment Only :

ATGP3738 has been replaced with a catalog number ATGP3651.

Molecular Weight :

17.4 kDa (157aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Amino Acid Sequence :

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P06804

Internal Search Terms :

TNF-a, Tumor necrosis factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin, ATGP3738, ATGP3651-10ug,ATGP3651-20ug,ATGP3651-50ug,ATGP3651-100ug,ATGP3651-250ug,ATGP3651-500ug,ATGP3651-1mg, ATGP3651-10,ATGP3651-20,ATGP3651-50,ATGP3651-100,ATGP3651-250,ATGP3651-500,ATGP3651-1,ATGP3651-10ug,ATGP3651-20ug,ATGP3651-50ug,ATGP3651-100ug,ATGP3651-250ug,ATGP3651-500ug,ATGP3651-1mg,ATGP3651-10,ATGP3651-20,ATGP3651-50,ATGP3651-100,ATGP3651-250,ATGP3651-500,ATGP3651-1

more info or order :

NKMAX product webpage