Recombinant mouse Lactate Dehydrogenase B/LDHB protein | NKMAX ATGP3643 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Lactate Dehydrogenase B/LDHB protein

catalog :

ATGP3643

quantity :

5 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Lactate Dehydrogenase B/LDHB protein

Domain :

1-334aa

Catalog number (SKU) :

ATGP3643

NCBI :

NP_032518

Description :

Ldhb, also known as L-lactate dehydrogenase B chain isoform Ldhb, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant mouse Ldhb protein, fused to His-tagging at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2, LDH heart subunit

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Read JA., et al. (2001) Proteins. 43(2):175-85.;; Butt AA., et al. (2002) AIDS Read. 12(7):317-21

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 150unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Cancer

Molecular Weight :

39 kDa (357aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 150 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMATLKEK LIASVADDEA AVPNNKITVV GVGQVGMACA ISILGKSLAD ELALVDVLED KLKGEMMDLQ HGSLFLQTPK IVADKDYSVT ANSKIVVVTA GVRQQEGESR LNLVQRNVNV FKFIIPQIVK YSPDCTIIVV SNPVDILTYV TWKLSGLPKH RVIGSGCNLD SARFRYLMAE KLGIHPSSCH GWILGEHGDS SVAVWSGVNV AGVSLQELNP EMGTDNDSEN WKEVHKMVVD SAYEVIKLKG YTNWAIGLSV ADLIESMLKN LSRIHPVSTM VKGMYGIENE VFLSLPCILN ARGLTSVINQ KLKDDEVAQL RKSADTLWDI QKDLKDL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P16125

Internal Search Terms :

L-lactate dehydrogenase B chain, Ldhb, L-lactate dehydrogenase B chain isoform Ldhb, AI790582, H-Ldh, Ldh-2, LDH-B, LDH-H, Ldh2,ATGP3643-10ug,ATGP3643-20ug,ATGP3643-50ug,ATGP3643-100ug,ATGP3643-250ug,ATGP3643-500ug,ATGP3643-1mg,ATGP3643-10,ATGP3643-20,ATGP3643-50,ATGP3643-100,ATGP3643-250,ATGP3643-500,ATGP3643-1

more info or order :

NKMAX product webpage